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A recommended positive control for IHC (frozen sections) is rat cerebellum.
Reconstitute with 50-100 µL of distilled water, and dilute further in 0.1M PBS with 1% BSA and 0.1% sodium azide.
The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles (McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).
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