Invitrogen

VGLL4 Recombinant Rabbit Monoclonal Antibody (16H12L24)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他7个VGLL4抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (2)
高级验证 (1)

VGLL4 Antibody in Immunocytochemistry (ICC/IF)

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VGLL4 Antibody (703012) in ICC/IF

Knockdown of VGLL4 was achieved by transfecting HCTt116 cells with specific siRNA (Silencer® select Product # s229766, s229764). Immunofluorescence analysis was performed on HCT116 cells (untransfected, panel a-d), transfected with VGLL4 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labeled with Anti-VGLL4 Recombinant Rabbit Monoclonal Antibody (Product # 703012, 1:100 dilution), followed by Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 c... View More

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VGLL4 Antibody — Antibody specificity was demonstrated by siRNA-mediated knockdown of the target protein. HCT116 cells were transfected with VGLL4 siRNA and a decrease in signal intensity was observed in immunofluorescence application using Anti-MBD1 Recombinant Rabbit Monoclonal Antibody (Product # 703012). {KD}

产品信息

703012

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

16H12L24

抗原

Peptides corresponding to human VGLL4 [1) aa6-aa23 2) aa235-aa253]

查看免疫原

偶联物

Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2784554

产品详细信息

This antibody is predicted to react with Monkey, Horse, Cat.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.