Invitrogen

Vimentin Polyclonal Antibody

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

4个已发表图片

7篇引用文献

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说明书

实验方案

技术支持

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Vimentin Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Vimentin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Vimentin Chicken Polyclonal Antibody (Product # PA1-16759) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Chicken IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A-11039) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic, cytoskeletal and nuclear localization. Panel e represents negative control, A-431 cells. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Vimentin Antibody in Western Blot (WB) — Western blot analysis was performed on Whole cell extracts (30 µg lysate) of HeLa (Lane 1), A-431 (Lane 2), SHSY-5Y (Lane 3), Hep G2 (Lane 4) and NIH/3T3 (Lane 5). The blot was probed with Anti-Vimentin Polyclonal Antibody (Product # PA1-16759, 1:2000 dilution) and detected by chemiluminescence using Goat anti-Chicken IgY (H+L) Secondary Antibody, HRP (Product # A16054, 0.25 µg/ml, 1:4000 dilution). A 54 kDa band corresponding to Vimentin was observed across all the cell lines positive for Vimentin (Lanes 1, 3 and 5), while this band was absent in the cell lines which do not express Vimentin protein (Lanes 2 and 4).

Vimentin Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Vimentin Polyclonal Antibody (Product # PA1-16759) showed expression of Vimentin in HeLa compared to A-431. {RE}

Vimentin Antibody in Immunohistochemistry (IHC) — Figure 1 Deletion of the leptin receptor (LepR) in brain endothelial and epithelial cells reduces leptin uptake by the brain . (A) Zs-Green reflecting expression of LepR was found in brain vessels (left and right panel) and in neurons of the VTA (right lower panel) of LepR-cre;Zs-Green mice. Nuclei are stained by DAPI (blue). Representative images are shown. Scale bar, 10 mum (left panel), 50 mum (right panels). (B and C) We used the reporter line HA-UPRT Fl to investigate in which cell types of the median eminence the Slco1c1-CreER T2 allele drives recombination. Immunofluorescent staining showed that HA-UPRT (red) was expressed in CD31-positive endothelial cells (green, B) of the cortex and mediobasal hypothalamus (C). There were no signs of recombination in vimentin-positive tanycytes (green, C). Scale bar, 50 mum. (D) Co-stainings of LepR (red) and CD31 (green) indicated that LepR was reduced in brain capillaries of Lepr beKO mice in comparison to LepR Fl controls. Capillaries in the cortex are depicted. (E) Relative LepR mRNA expression in primary brain endothelial cells. Values represent means +- SEM. Unpaired t test, *p < 0.05 (n = 3 mice/group). (F) Leptin uptake in the mediobasal hypothalamus (MBH), ventral tegmental area (VTA), cortex, cerebrospinal fluid (CSF), and the rest of the brain was reduced in LepR beKO mice compared to LepR Fl animals. Mice were perfused with 125 I-leptin for 30 min followed by a washout phase of 10 min. Values represent means +- SEM. Two-

产品信息

PA1-16759

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:2,000-1:20,000	-
免疫组化 (IHC)	-	查看6篇已发表文章 6篇已发表文章
免疫细胞化学 (ICC/IF)	1:200-1:5000	查看1篇已发表文章 1篇已发表文章

产品规格
种属反应	Human, Mouse, Rat
已发表种属	Human, Mouse
宿主/亚型	Chicken / IgY
分类	Polyclonal
类型	Antibody
抗原	The immunogen used to generate our antibody was recombinant vimentin purified from E. coli.
偶联物	Unconjugated
形式	Liquid
纯化类型	IgY fraction
保存液	PBS
内含物	0.02% sodium azide
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2257294

靶标信息

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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PA1-16759 was used in Immunohistochemistry-immunofluorescence to show biomimetic electromechanical stimulation of adult cardiac tissue can maintain structural, functional and transcriptional properties.