Invitrogen

Vimentin Polyclonal Antibody

Vimentin Antibody in Immunocytochemistry (ICC/IF)

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Vimentin Antibody (PA5-27231) in ICC/IF

Immunofluorescence analysis of Vimentin was performed using 70 confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Vimentin Polyclonal Antibody (Product # PA5-27231) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Pro... View More

Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Vimentin Antibody in Immunoprecipitation (IP)

Vimentin Antibody in Immunohistochemistry (IHC)

Vimentin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Vimentin Antibody in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation (ICC/IF, IHC, IP)

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产品信息

PA5-27231

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000-1:50,000

查看11篇已发表文章 11篇已发表文章

免疫组化 (IHC)

查看7篇已发表文章 7篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫组化（冰冻） (IHC (F))

Assay-dependent

查看2篇已发表文章 2篇已发表文章

免疫组织化学（悬浮细胞） (IHC (Free))

Assay-dependent

免疫细胞化学 (ICC/IF)

1:100-1:1,000

查看8篇已发表文章 8篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫沉淀 (IP)

1:100-1:500

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Chicken, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.16 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 1% BSA, 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2544707

产品详细信息

Recommended positive controls: 293T, HeLa, MDA-MB-231, U87-MG, SK-N-SH, IMR32, SK-N-AS, NIH-3T3, A549.

Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (85%), Dog (98%), Chicken (92%), Chimpanzee (99%), Bovine (98%), Guinea pig (97%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Epigenetic upregulation of MNAT1 by SMYD2 is linked to PI3K/AKT activation and tumorigenesis of pancreatic adenocarcinoma.

Histology and histopathology

Xu Z,Liu Y,Pan Z,Qin L

PA5-27231 was used in Western Blot to investigate the roles of SMYD2 and its interacted molecules in pancreatic adenocarcinoma (PAAD).

Thu Feb 01 00:00:00 UTC 2024

Evaluation of Epithelial-Mesenchymal Transition Markers in Autoimmune Thyroid Diseases.

International journal of molecular sciences

Sacristán-Gómez P,Serrano-Somavilla A,Castro-Espadas L,Sánchez de la Blanca Carrero N,Sampedro-Núñez M,Muñoz-De-Nova JL,Molina-Jiménez F,Rosell A,Marazuela M,Martínez-Hernández R

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Immunohistochemistry

Wed Feb 08 00:00:00 UTC 2023

Evaluation of Epithelial-Mesenchymal Transition Markers in Autoimmune Thyroid Diseases.

International journal of molecular sciences

Sacristán-Gómez P,Serrano-Somavilla A,Castro-Espadas L,Sánchez de la Blanca Carrero N,Sampedro-Núñez M,Muñoz-De-Nova JL,Molina-Jiménez F,Rosell A,Marazuela M,Martínez-Hernández R

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Immunohistochemistry

Wed Feb 08 00:00:00 UTC 2023

Evaluation of Epithelial-Mesenchymal Transition Markers in Autoimmune Thyroid Diseases.

International journal of molecular sciences

Sacristán-Gómez P,Serrano-Somavilla A,Castro-Espadas L,Sánchez de la Blanca Carrero N,Sampedro-Núñez M,Muñoz-De-Nova JL,Molina-Jiménez F,Rosell A,Marazuela M,Martínez-Hernández R

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Immunohistochemistry

Wed Feb 08 00:00:00 UTC 2023

Evaluation of Epithelial-Mesenchymal Transition Markers in Autoimmune Thyroid Diseases.

International journal of molecular sciences

Sacristán-Gómez P,Serrano-Somavilla A,Castro-Espadas L,Sánchez de la Blanca Carrero N,Sampedro-Núñez M,Muñoz-De-Nova JL,Molina-Jiménez F,Rosell A,Marazuela M,Martínez-Hernández R

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Immunohistochemistry

Wed Feb 08 00:00:00 UTC 2023

MIR503HG silencing promotes endometrial stromal cell progression and metastasis and suppresses apoptosis in adenomyosis by activating the Wnt/β‑catenin pathway via targeting miR‑191.

Experimental and therapeutic medicine

Xu X,Cai B,Liu Y,Liu R,Li J

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Immunocytochemistry

Wed Mar 01 00:00:00 UTC 2023

MIR503HG silencing promotes endometrial stromal cell progression and metastasis and suppresses apoptosis in adenomyosis by activating the Wnt/β‑catenin pathway via targeting miR‑191.

Experimental and therapeutic medicine

Xu X,Cai B,Liu Y,Liu R,Li J

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Immunocytochemistry

Wed Mar 01 00:00:00 UTC 2023

Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.

Haematologica

Michalik S,Siegerist F,Palankar R,Franzke K,Schindler M,Reder A,Seifert U,Cammann C,Wesche J,Steil L,Hentschker C,Gesell-Salazar M,Reisinger E,Beer M,Endlich N,Greinacher A,Völker U

PA5-27231 was used in Immunocytochemistry to conclude that these differences in impurities together with EDTAinduced capillary leakage might contribute to the higher incidence rate of VITT associated with ChAdOx1 nCoV-19 compared to Ad26.COV2.S.

Fri Apr 01 00:00:00 UTC 2022

Brown Adipose Tissue Sheds Extracellular Vesicles That Carry Potential Biomarkers of Metabolic and Thermogenesis Activity Which Are Affected by High Fat Diet Intervention.

International journal of molecular sciences

Camino T,Lago-Baameiro N,Sueiro A,Bravo SB,Couto I,Santos FF,Baltar J,Casanueva FF,Pardo M

PA5-27231 was used in Western Blotting to isolate batosomes from lean and obese Sprague-Dawley rats, and to establish reference proteome maps.

Fri Sep 16 00:00:00 UTC 2022

CD44 alternative splicing senses intragenic DNA methylation in tumors via direct and indirect mechanisms.

Nucleic acids research

Batsché E,Yi J,Mauger O,Kornobis E,Hopkins B,Hanmer-Lloyd C,Muchardt C

PA5-27231 was used in Western Blot to explore the possibility of the impact of DNA methylation on alternative splicing that favors tumor progression.

Mon Jun 21 00:00:00 UTC 2021

CD44 alternative splicing senses intragenic DNA methylation in tumors via direct and indirect mechanisms.

Nucleic acids research

Batsché E,Yi J,Mauger O,Kornobis E,Hopkins B,Hanmer-Lloyd C,Muchardt C

PA5-27231 was used in Western Blot to explore the possibility of the impact of DNA methylation on alternative splicing that favors tumor progression.

Mon Jun 21 00:00:00 UTC 2021

Mitotic Activation Around Wound Edges and Epithelialization Repair in UVB-Induced Capsular Cataracts.

Investigative ophthalmology & visual science

Wei Z,Hao C,Srinivasagan R,Wu H,Chen JK,Fan X

PA5-27231 was used in Immunohistochemistry-immunofluorescence to demonstrate that normally quiescent lens epithelial cells can be reactivated for epithelialization repair in response to UV-induced damage.

Wed Dec 01 00:00:00 UTC 2021

Identification of the Molecular Basis of Nanocurcumin-Induced Telocyte Preservation within the Colon of Ulcerative Colitis Rat Model.

Mediators of inflammation

Arafat EA,Marzouk RE,Mostafa SA,Hamed WHE

PA5-27231 was used in Immunohistochemistry to examine the effect of nanocurcumin (NC) on the morphometric and immunohistochemical characterization of telocytes in the ulcerative colitis (UC) rat model.

Mon Jan 24 00:00:00 UTC 2022

Identification of the Molecular Basis of Nanocurcumin-Induced Telocyte Preservation within the Colon of Ulcerative Colitis Rat Model.

Mediators of inflammation

Arafat EA,Marzouk RE,Mostafa SA,Hamed WHE

Mon Jan 24 00:00:00 UTC 2022

Establishment and Characterization of a Novel Dedifferentiated Chondrosarcoma Cell Line DDCS2.

Cancer control : journal of the Moffitt Cancer Center

Li X,Dean DC,Ferreira A,Nelson SD,Hornicek FJ,Yu S,Duan Z

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Western Blot

Fri Jan 21 00:00:00 UTC 2022

Establishment and Characterization of a Novel Dedifferentiated Chondrosarcoma Cell Line DDCS2.

Cancer control : journal of the Moffitt Cancer Center

Li X,Dean DC,Ferreira A,Nelson SD,Hornicek FJ,Yu S,Duan Z

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Western Blot

Fri Jan 21 00:00:00 UTC 2022

D-Propranolol Impairs EGFR Trafficking and Destabilizes Mutant p53 Counteracting AKT Signaling and Tumor Malignancy.

Cancers

Barra J,Cerda-Infante J,Sandoval L,Gajardo-Meneses P,Henriquez JF,Labarca M,Metz C,Venegas J,Retamal C,Oyanadel C,Cancino J,Soza A,Cuello MA,Roa JC,Montecinos VP,Gonzalez A

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Immunohistochemistry

Tue Jul 20 00:00:00 UTC 2021

Ginsenoside Rg3 inhibits osteosarcoma progression by reducing circ_0003074 expression in a miR-516b-5p/KPNA4-dependent manner.

Journal of orthopaedic surgery and research

Wang T,Zhang C,Wang S

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Immunohistochemistry

Mon Dec 20 00:00:00 UTC 2021

E2F5 Promotes the Malignancy of Ovarian Cancer Via the Regulation of Hippo and Wnt Pathways.

Genetic testing and molecular biomarkers

Malgundkar SH,Burney I,Al Moundhri M,Al Kalbani M,Lakhtakia R,Okamoto A,Tamimi Y

PA5-27231 was used in Western Blot to conclude that E2F5 promotes ovarian carcinogenesis via the regulation of Hippo and Wnt pathways.

Mon Mar 01 00:00:00 UTC 2021

Differential expression and inhibitory effects of aquaporins on the development of adenomyosis.

Molecular medicine reports

Jia L,Liu Y,Han Y,Zhou X,Wang F

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Immunocytochemistry

Sun Nov 01 00:00:00 UTC 2020

Differential expression and inhibitory effects of aquaporins on the development of adenomyosis.

Molecular medicine reports

Jia L,Liu Y,Han Y,Zhou X,Wang F

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Immunocytochemistry

Sun Nov 01 00:00:00 UTC 2020

FAT4 silencing promotes epithelial-to-mesenchymal transition and invasion via regulation of YAP and β-catenin activity in ovarian cancer.

BMC cancer

Malgundkar SH,Burney I,Al Moundhri M,Al Kalbani M,Lakhtakia R,Okamoto A,Tamimi Y

PA5-27231 was used in Western Blotting to examine the role of FAT4 in proliferation and metastasis, and its mechanisms of interaction in these processes.

Mon May 04 00:00:00 UTC 2020

FAT4 silencing promotes epithelial-to-mesenchymal transition and invasion via regulation of YAP and β-catenin activity in ovarian cancer.

BMC cancer

Malgundkar SH,Burney I,Al Moundhri M,Al Kalbani M,Lakhtakia R,Okamoto A,Tamimi Y

PA5-27231 was used in Western Blotting to examine the role of FAT4 in proliferation and metastasis, and its mechanisms of interaction in these processes.

Mon May 04 00:00:00 UTC 2020

Periconceptional alcohol exposure causes female-specific perturbations to trophoblast differentiation and placental formation in the rat.

Development (Cambridge, England)

Kalisch-Smith JI,Steane SE,Simmons DG,Pantaleon M,Anderson ST,Akison LK,Wlodek ME,Moritz KM

PA5-27231 was used in Immunohistochemistry to show periconceptional alcohol exposure causes reduced placental volume at embryonic day 15 due to affecting trophoblast differentiation, embryo-uterine communication, and formation of the placental vasculature.

Mon Jun 10 00:00:00 UTC 2019

Citrullination Controls Dendritic Cell Transdifferentiation into Osteoclasts.

Journal of immunology (Baltimore, Md. : 1950)

Krishnamurthy A,Ytterberg AJ,Sun M,Sakuraba K,Steen J,Joshua V,Tarasova NK,Malmström V,Wähämaa H,Réthi B,Catrina AI

PA5-27231 was used in Immunocytochemistry-immunoflourescence to investigate whether protein citrullination and RA-specific anti-citrullinated protein could regulate human blood-derived DC-OC transdifferentiation.

Sat Jun 01 00:00:00 UTC 2019

Vimentin acetylation is involved in SIRT5-mediated hepatocellular carcinoma migration.

American journal of cancer research

Guo D,Song X,Guo T,Gu S,Chang X,Su T,Yang X,Liang B,Huang D

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Western Blot

Wed Sep 30 00:00:00 UTC 2020

Vimentin acetylation is involved in SIRT5-mediated hepatocellular carcinoma migration.

American journal of cancer research

Guo D,Song X,Guo T,Gu S,Chang X,Su T,Yang X,Liang B,Huang D

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Western Blot

Wed Sep 30 00:00:00 UTC 2020

Vimentin acetylation is involved in SIRT5-mediated hepatocellular carcinoma migration.

American journal of cancer research

Guo D,Song X,Guo T,Gu S,Chang X,Su T,Yang X,Liang B,Huang D

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Western Blot

Wed Sep 30 00:00:00 UTC 2020

Vimentin acetylation is involved in SIRT5-mediated hepatocellular carcinoma migration.

American journal of cancer research

Guo D,Song X,Guo T,Gu S,Chang X,Su T,Yang X,Liang B,Huang D

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Western Blot

Wed Sep 30 00:00:00 UTC 2020

Forkhead box C2 promotes the invasion ability of human trophoblast cells through Hedgehog (Hh) signaling pathway.

Cell biology international

Zhang Y,Zhang Y

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Western Blot

Sun Jul 01 00:00:00 UTC 2018

Myostatin promotes the epithelial-to-mesenchymal transition of the dermomyotome during somitogenesis.

Developmental dynamics : an official publication of the American Association of Anatomists

Zhou Y,Zhang Y,Zhu D

PA5-27231 was used in Immunohistochemistry (Frozen) to suggest that MSTN regulates myogenesis by promoting EMT during somitogenesis and provide novel insights into the functions of MSTN during early embryonic myogenesis.

Sat Dec 01 00:00:00 UTC 2018

Molecular oncology

Kryza T,Silva LM,Bock N,Fuhrman-Luck RA,Stephens CR,Gao J,Samaratunga H,Lawrence MG,Hooper JD,Dong Y,Risbridger GP,Clements JA

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Western Blot

Sun Oct 01 00:00:00 UTC 2017

Cytosolic Hsp90α and its mitochondrial isoform Trap1 are differentially required in a breast cancer model.

Oncotarget

Vartholomaiou E,Madon-Simon M,Hagmann S,Mühlebach G,Wurst W,Floss T,Picard D

Published figure using Vimentin polyclonal antibody (Product # PA5-27231) in Western Blot

Tue Mar 14 00:00:00 UTC 2017

Doxorubicin and cisplatin induce apoptosis in ovarian stromal cells obtained from cryopreserved human ovarian tissue.

Future oncology (London, England)

Fabbri R,Macciocca M,Vicenti R,Paradisi R,Klinger FG,Pasquinelli G,Spisni E,Seracchioli R,Papi A

PA5-27231 was used in immunocytochemistry to analyze the induction of apoptosis in ovarian stromal cells obtained from cryopreserved human ovarian tissue by cisplatin and doxorubicin