Invitrogen

Vimentin Monoclonal Antibody (V9), FITC, eBioscience™

Vimentin Antibody in Immunocytochemistry (ICC/IF)

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Vimentin Antibody (11-9897-82) in ICC/IF

Immunocytochemistry of fixed and permeabilized C6 cells using 1 µg/mL of Mouse IgG1 Isotype Control FITC (Product # 11-4714-42) (left) or 1 µg/mL of Anti-Vimentin FITC (right). Nuclei are counterstained with DAPI.

Vimentin Antibody in Flow Cytometry (Flow)

Vimentin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Vimentin Antibody in Immunohistochemistry (IHC)

Product Image Gallery

产品信息

11-9897-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看6篇已发表文章 6篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

Assay-Dependent

查看1篇已发表文章 1篇已发表文章

免疫组化（冰冻） (IHC (F))

Assay-Dependent

免疫细胞化学 (ICC/IF)

Assay-Dependent

查看6篇已发表文章 6篇已发表文章

流式细胞分析 (Flow)

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Dog, Chicken, Human, Rat

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10597000

产品详细信息

Description: The V9 monoclonal antibody recognizes human Vimentin, a 57 kDa protein that functions as a structural component of intermediate filaments. Vimentin is expressed in cells derived from the mesenchyme but also in specific populations such as radial glia and immature glial cells, pancreatic precursor cells. It is proposed to be a marker of cardiac differentiation. In neural cells, vimentin expression is gradually replaced by neurofilaments. Reports have also shown surface expression of vimentin on activated macrophages, platelets, as well as apoptotic T cells and neutrophils.

This antibody also recognizes canine (dog), rat and chicken vimentin but does not recognize mouse vimentin.

Applications Reported: This V9 antibody has been reported for use in immunocytochemistry and immunohistochemical staining of frozen (IHC-F) and formalin-fixed paraffin embedded tissue sections (IHC-P).

Applications Tested: This V9 antibody has been tested by immunofluorescent staining of paraformaldehyde fixed and permeabilized cells. This can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

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Matrix stiffness induces epithelial-to-mesenchymal transition via Piezo1-regulated calcium flux in prostate cancer cells.

iScience

Lopez-Cavestany M,Hahn SB,Hope JM,Reckhorn NT,Greenlee JD,Schwager SC,VanderBurgh JA,Reinhart-King CA,King MR

Published figure using Vimentin monoclonal antibody (Product # 11-9897-82) in Western Blot

Fri Apr 21 00:00:00 UTC 2023

Pericardial malignant mesothelioma diagnosed in a dog by immunocytochemistry of the pericardial fluid: a case report.

BMC veterinary research

Choi EW

11-9897-82 was used in Immunocytochemistry to show that E-cadherin and calretinin can be used for the differential diagnosis of reactive mesothelial cells, malignant mesothelioma, and adenocarcinoma in dogs.

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Aspirin increases the efficacy of gemcitabine in pancreatic cancer by modulating the PI3K/AKT/mTOR signaling pathway and reversing epithelial‑mesenchymal transition.

Oncology letters

Zhou H,Yun X,Shu Y,Xu K

Published figure using Vimentin monoclonal antibody (Product # 11-9897-82) in Western Blot

Wed Mar 01 00:00:00 UTC 2023

Theranostics application of tumor-initiating cell probe TiY in non-small cell lung cancer.

Theranostics

Lee YA,Lek CCJ,Rong G,Wu Z,Shathishwaran S,Lee JHJ,Tam WL,Wuestefeld T,Park SJ,Jung S,Kim B,Kang NY,Chang YT

Published figure using Vimentin monoclonal antibody (Product # 11-9897-82) in Western Blot

Mon Mar 20 00:00:00 UTC 2023

Theranostics application of tumor-initiating cell probe TiY in non-small cell lung cancer.

Theranostics

Lee YA,Lek CCJ,Rong G,Wu Z,Shathishwaran S,Lee JHJ,Tam WL,Wuestefeld T,Park SJ,Jung S,Kim B,Kang NY,Chang YT

Published figure using Vimentin monoclonal antibody (Product # 11-9897-82) in Western Blot

Mon Mar 20 00:00:00 UTC 2023

Conjugation of VEGFR1/R2-targeting peptide with gold nanoparticles to enhance antiangiogenic and antitumoral activity.

Journal of nanobiotechnology

Zanjanchi P,Asghari SM,Mohabatkar H,Shourian M,Shafiee Ardestani M

Published figure using Vimentin monoclonal antibody (Product # 11-9897-82) in Western Blot

Tue Jan 04 00:00:00 UTC 2022

Development of human-derived, three-dimensional respiratory epithelial tissue constructs with perfusable microvasculature on a high-throughput microfluidics screening platform.

Biofabrication

Jung O,Tung YT,Sim E,Chen YC,Lee E,Ferrer M,Song MJ

11-9897-82 was used in Flow Cytometry to develop two novel vascularized, LRT multi-chip models for the alveoli and the small airway.

Mon Feb 14 00:00:00 UTC 2022

Characterization of epithelial cells, connective tissue cells and immune cells in human upper airway mucosa by immunofluorescence multichannel image cytometry: a pilot study.

Histochemistry and cell biology

Giotakis AI,Dudas J,Glueckert R,Dejaco D,Ingruber J,Fleischer F,Innerhofer V,Pinggera L,Bektic-Tadic L,Gabriel SAM,Riechelmann H

Published figure using Vimentin monoclonal antibody (Product # 11-9897-82) in Immunohistochemistry

Mon Mar 01 00:00:00 UTC 2021

Characterization of epithelial cells, connective tissue cells and immune cells in human upper airway mucosa by immunofluorescence multichannel image cytometry: a pilot study.

Histochemistry and cell biology

Giotakis AI,Dudas J,Glueckert R,Dejaco D,Ingruber J,Fleischer F,Innerhofer V,Pinggera L,Bektic-Tadic L,Gabriel SAM,Riechelmann H

Published figure using Vimentin monoclonal antibody (Product # 11-9897-82) in Immunohistochemistry

Mon Mar 01 00:00:00 UTC 2021

MicroRNA-384 inhibits nasopharyngeal carcinoma growth and metastasis via binding to Smad5 and suppressing the Wnt/β-catenin axis.

Cytotechnology

Zeng X,Liao H,Wang F

Published figure using Vimentin monoclonal antibody (Product # 11-9897-82) in Western Blot

Thu Apr 01 00:00:00 UTC 2021

Defining tumor-associated vascular heterogeneity in pediatric high-grade and diffuse midline gliomas.

Acta neuropathologica communications

Wei X,Meel MH,Breur M,Bugiani M,Hulleman E,Phoenix TN

11-9897-82 was used in Immunohistochemistry (Paraffin) to characterise and directly compare tumor-associated vasculature in cortical pediatric high-grade gliomas and diffuse midline gliomas.

Mon Aug 23 00:00:00 UTC 2021

Omentin-1 is associated with atrial fibrillation in patients with cardiac valve disease.

BMC cardiovascular disorders

Chen Y,Liu F,Han F,Lv L,Tang CE,Xie Z,Luo F

Published figure using Vimentin monoclonal antibody (Product # 11-9897-82) in Immunocytochemistry

Wed May 06 00:00:00 UTC 2020

Omentin-1 is associated with atrial fibrillation in patients with cardiac valve disease.

BMC cardiovascular disorders

Chen Y,Liu F,Han F,Lv L,Tang CE,Xie Z,Luo F

Published figure using Vimentin monoclonal antibody (Product # 11-9897-82) in Immunocytochemistry

Wed May 06 00:00:00 UTC 2020

GMP-compatible and xeno-free cultivation of mesenchymal progenitors derived from human-induced pluripotent stem cells.

Stem cell research & therapy

McGrath M,Tam E,Sladkova M,AlManaie A,Zimmer M,de Peppo GM

Published figure using Vimentin monoclonal antibody (Product # 11-9897-82) in Immunocytochemistry

Fri Jan 11 00:00:00 UTC 2019

Levels of hepatic Th17 cells and regulatory T cells upregulated by hepatic stellate cells in advanced HBV-related liver fibrosis.

Journal of translational medicine

Li X,Su Y,Hua X,Xie C,Liu J,Huang Y,Zhou L,Zhang M,Li X,Gao Z

Published figure using Vimentin monoclonal antibody (Product # 11-9897-82) in Immunofluorescence

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Chronic macrophage migration inhibitory factor exposure induces mesenchymal epithelial transition and promotes gastric and colon cancers.

PloS one

Morris KT,Nofchissey RA,Pinchuk IV,Beswick EJ

11-9897 was used in Flow cytometry/Cell sorting to study how the MIF promotes mesenchymal epithelial transition, cell transformation and tumorigenesis in GI cancers, and thus may be an important link between chronic inflammation and tumorigenesis.

Tue Jan 20 00:00:00 UTC 2015

Expression of the progenitor marker NG2/CSPG4 predicts poor survival and resistance to ionising radiation in glioblastoma.

Acta neuropathologica

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