Invitrogen

WDR45B Polyclonal Antibody

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WDR45B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of WDR45B in paraffin embedded Human cervical cancer tissue using WDR45B Polyclonal Antibody (Product # PA5-50864) at a 1:200 dilution.

WDR45B Antibody in Western Blot (WB) — Fig. 7 Neurological defects in neuron-specific Wipi3 cKO mice. a Western blot analysis of brain lysates. The asterisk indicates a nonspecific band. Wipi3 cKO mice at 18-weeks were compared with Atg7 cKO mice at 5-weeks, as they showed equivalent disease severity. b - d Abnormal motor performance in Wipi3 cKO mice at 10-weeks. The limb-clasping reflex was observed ( b ). The footprint assay indicated a motor deficit ( c ). In ( d ), the time the indicated mice remained on the rod was measured. Data are shown as the mean +- SD ( n = 3). e - g Cryosections of the indicated cerebellum were immunostained for the Purkinje cell marker calbindin (green), the glial cell marker GFAP (red), and DAPI (blue). Bars = 200 um ( e ) and 20 um ( g ). In ( f ), the number of Purkinje cells in the first fissure of the cerebellum is shown. Data are shown as the mean +- SD ( n = 3). h - m Comparisons of morphology between WT, Wipi3 cKO mice (10-weeks), and Atg7 cKO mice (5-weeks). In ( h ), cryosections of the cerebellum immunostained with anti-calbindin and anti-p62 antibodies. Bar = 10 um. ( i ) Morphology of Golgi in cerebella. Small fragmented and swollen rod-shaped Golgi membranes were observed in the Purkinje cells from Wipi3 cKO mice. Bars = 0.5 um. j Immunostaining of calbindin and ubiquitin (Ub) using cryosections of the cerebellum. Bars = 20 um. k , l The accumulation of fibrils was observed in Purkinje cells from Wipi3 cKO mice. Bars = 0.5 um. Orange and red arrows indicate naked fibril

产品信息

PA5-50864

应用	建议稀释比	已发表文章
免疫组化（石蜡） (IHC (P))	1:100-1:200	-
免疫组化 (IHC)	-	查看1篇已发表文章 1篇已发表文章
免疫印迹 (WB)	-	查看1篇已发表文章 1篇已发表文章

产品规格
种属反应	Human
已发表种属	Mouse
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Synthetic peptide of human WD repeat domain 45B
偶联物	Unconjugated
形式	Liquid
浓度	2.5 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7.4, with 40% glycerol
内含物	0.05% sodium azide
保存条件	-20°C
RRID	AB_2636316

产品详细信息

The antibody detects endogenous levels of total WDR45B protein.

靶标信息

This gene encodes a member of the WIPI or SVP1 family of WD40 repeat-containing proteins. The protein contains seven WD40 repeats that are thought to fold into a beta-propeller structure that mediates protein-protein interactions, and a conserved motif for interaction with phospholipids. The human genome contains several pseudogenes of this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Wipi3 is essential for alternative autophagy and its loss causes neurodegeneration.

Nature communications

Yamaguchi H,Honda S,Torii S,Shimizu K,Katoh K,Miyake K,Miyake N,Fujikake N,Sakurai HT,Arakawa S,Shimizu S

PA5-50864 was used in Immunohistochemistry-immunofluorescence to identify Wipi3 as a molecule essential for alternative autophagy, but which plays minor roles in canonical autophagy.

Tue Oct 20 00:00:00 EDT 2020

Wipi3 is essential for alternative autophagy and its loss causes neurodegeneration.

Nature communications

Yamaguchi H,Honda S,Torii S,Shimizu K,Katoh K,Miyake K,Miyake N,Fujikake N,Sakurai HT,Arakawa S,Shimizu S

PA5-50864 was used in Immunohistochemistry-immunofluorescence to identify Wipi3 as a molecule essential for alternative autophagy, but which plays minor roles in canonical autophagy.

Tue Oct 20 00:00:00 EDT 2020

生物信息学

蛋白别名: WD repeat domain phosphoinositide-interacting protein 3; WD repeat protein 45-like; WD repeat-containing protein 45-like; WD repeat-containing protein 45B; WDR45-like protein; WIPI-3; WIPI49-like protein

基因别名: WDR45B; WDR45L; WIPI-3; WIPI3

UniProt ID: (Human) Q5MNZ6

Entrez Gene ID: (Human) 56270

功能

autophagosome assembly mitophagy protein lipidation protein localization to pre-autophagosomal structure nucleophagy

参与通路

phosphatidylinositol-3-phosphate binding phosphatidylinositol-3,5-bisphosphate binding

WDR45B Polyclonal Antibody

产品信息

PA5-50864

免疫组化（石蜡） (IHC (P))

免疫组化 (IHC)

免疫印迹 (WB)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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