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WNK1 Antibody (711356) in ICC/IF
For immunofluorescence analysis, A549 cells were fixed and permeabilized for detection of endogenous WNK1 using Anti- WNK1 Recombinant Rabbit Polyclonal Antibody (Product # 711356, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of WNK1 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938).... View More
产品信息
711356
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Antibody
克隆号
3HCLC
抗原
Peptides corresponding to Human WNK1 (aa 2338-2355, 45-56)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2633169
产品详细信息
This antibody is predicted to react with Monkey, Rabbit, Pig and Mouse
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
靶标信息
WNK1 serine/threonine kinase is a member of the WNK subfamily. WNK1 may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. WNK plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Also, acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D, and SGK1. WNK localizes to a segment of kidney involved in salt, potassium, and pH homeostasis, the distal nephron. Mutations in the WNK gene result in Pseudohypaldosteronism and Neuropathy.
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生物信息学
蛋白别名: Erythrocyte 65 kDa protein; hWNK1; Kinase deficient protein; MGC163339; MGC163341; p65; prostate-derived sterile 20-like kinase; Protein kinase lysine-deficient 1; Protein kinase with no lysine 1; protein phosphatase 1, regulatory subunit 167; Serine/threonine-protein kinase WNK1; serine/threonine-protein kinase WNK1 1; serine/threonine-protein kinase WNK1 2; With no K; WNK lysine deficient protein kinase 1 isoform
基因别名: HSAN2; HSN2; KDP; KIAA0344; p65; PPP1R167; PRKWNK1; PSK; WNK1
UniProt ID: (Human) Q9H4A3
Entrez Gene ID: (Human) 65125
magnesium ion binding
protein serine/threonine kinase activity
protein binding
ATP binding
chloride channel inhibitor activity
potassium channel inhibitor activity
protein kinase binding
phosphatase binding
protein serine/threonine kinase inhibitor activity
non-receptor serine/threonine protein kinase
positive regulation of systemic arterial blood pressure
protein phosphorylation
ion transport
negative regulation of phosphatase activity
peptidyl-threonine phosphorylation
positive regulation of ion transmembrane transporter activity
intracellular signal transduction
protein autophosphorylation
neuron development
regulation of cellular process
negative regulation of protein serine/threonine kinase activity
negative regulation of pancreatic juice secretion
regulation of ion homeostasis
