Invitrogen

XIAP Polyclonal Antibody

XIAP Antibody in Immunocytochemistry (ICC/IF)

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XIAP Antibody (PA5-29253) in ICC/IF

Immunofluorescence analysis of XIAP was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with anti-XIAP Rabbit Polyclonal Antibody (Product # PA5-29253) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Pro... View More

XIAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

XIAP Antibody in Immunohistochemistry (IHC)

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XIAP Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of XIAP was observed in mouse lung as compared to mouse brain using XIAP Polyclonal Antibody (Product # PA5-29253) in western blot. {KD}

产品信息

PA5-29253

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse, Non-human primate

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the center region of human XIAP. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 10% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2546729

产品详细信息

Recommended positive controls: HeLa, Mouse brain.

Predicted reactivity: Mouse (90%), Rat (88%), Dog (85%), Pig (89%), Rabbit (89%), Bovine (87%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Suppression of EGFR/PKC-δ/NF-κB Signaling Associated With Imipramine-Inhibited Progression of Non-Small Cell Lung Cancer.

Frontiers in oncology

Yueh PF,Lee YH,Chiang IT,Chen WT,Lan KL,Chen CH,Hsu FT

Published figure using XIAP polyclonal antibody (Product # PA5-29253) in Immunohistochemistry

Thu Apr 28 00:00:00 UTC 2022

Suppression of EGFR/PKC-δ/NF-κB Signaling Associated With Imipramine-Inhibited Progression of Non-Small Cell Lung Cancer.

Frontiers in oncology

Yueh PF,Lee YH,Chiang IT,Chen WT,Lan KL,Chen CH,Hsu FT

Published figure using XIAP polyclonal antibody (Product # PA5-29253) in Immunohistochemistry

Thu Apr 28 00:00:00 UTC 2022

HtrA4 Protease Promotes Chemotherapeutic-Dependent Cancer Cell Death.

Cells

Wenta T,Rychlowski M,Jarzab M,Lipinska B

Published figure using XIAP polyclonal antibody (Product # PA5-29253) in Western Blot

Fri Sep 20 00:00:00 UTC 2019

Fluoxetine Induces Apoptosis through Extrinsic/Intrinsic Pathways and Inhibits ERK/NF-κB-Modulated Anti-Apoptotic and Invasive Potential in Hepatocellular Carcinoma Cells In Vitro.

International journal of molecular sciences

Chen WT,Hsu FT,Liu YC,Chen CH,Hsu LC,Lin SS

PA5-29253 was used in Western Blotting to verify the effects of fluoxetine on dysregulation of apoptosis and invasive potential in human hepatocellular carcinoma (HCC) SK-Hep1 and Hep3B cells.

Mon Feb 11 00:00:00 UTC 2019

Protein Kinase B and Extracellular Signal-Regulated Kinase Inactivation is Associated with Regorafenib-Induced Inhibition of Osteosarcoma Progression In Vitro and In Vivo.

Journal of clinical medicine

Pan PJ,Liu YC,Hsu FT

PA5-29253 was used in Western Blotting to study the efficacy and molecular mechanism of regorafenib on osteoscarcoma in vitro and in vivo.

Mon Jun 24 00:00:00 UTC 2019

Magnolol Induces Apoptosis and Inhibits ERK-modulated Metastatic Potential in Hepatocellular Carcinoma Cells.

In vivo (Athens, Greece)

Kuan LY,Chen WL,Chen JH,Hsu FT,Liu TT,Chen WT,Wang KL,Chen WC,Liu YC,Wang WS

PA5-29253 was used in Western Blotting to evaluate the anti-cancer effect of magnolol in hepatocellular carcinoma cells in vitro.

Mon Jan 28 00:00:00 UTC 2019

生物信息学

蛋白别名: Baculoviral IAP repeat-containing protein 4; BIR4; E3 ubiquitin-protein ligase XIAP; HILP; IAP homolog A; IAP-3; IAP-like protein; ILP; Inhibitor of apoptosis protein 3; RING-type E3 ubiquitin transferase XIAP; RP1-315G1.5; X-linked IAP; X-linked inhibitor of apoptosis protein; X-linked inhibitor of apoptosis, E3 ubiquitin protein ligase

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基因别名: 1110015C02Rik; Aipa; API3; BIRC4; hIAP-3; hIAP3; IAP-3; IAP3; ILP-1; ILP1; MIHA; XIAP; XLP2

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UniProt ID: (Human) P98170, (Mouse) Q60989

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Entrez Gene ID: (Human) 331, (Mouse) 11798

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