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Invitrogen
XPF Polyclonal Antibody
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XPF Antibody (PA5-117118) in WB
Western blot analysis of XPF in extracts of various cell lines. Samples were incubated with XPF Polyclonal antibody (Product # PA5-117118) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
产品信息
PA5-117118
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence within amino acids 513-782 of human ERCC4 (NP_0052271)
偶联物
形式
Liquid
浓度
1.49 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Positive Samples: U-251MG, HT-1080
Immunogen sequence: GYRREISSSP ESCPEEIKHE EFDVNLSSDA AFGILKEPLT IIHPLLGCSD PYALTRVLHE VEPRYVVLYD AELTFVRQLE IYRASRPGKP LRVYFLIYGG STEEQRYLTA LRKEKEAFEK LIREKASMVV PEEREGRDET NLDLVRGTAS ADVSTDTRKA GGQEQNGTQQ SIVVDMREFR SELPSLIHRR GIDIEPVTLE VGDYILTPEM CVERKSISDL IGSLNNGRLY SQCISMSRYY KRPVLLIEFD PSKPFSLTSR GALFQEISSN
靶标信息
The structure specific ERCC 1 / XPF endonuclease complex is implicated in the repair of two distinct types of lesions in DNA: NER for UV induced lesions and bulky chemical adducts; and recombination repair of the very genotoxic interstrand cross links. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells, XPG cleaves 3' of the DNA lesion while the ERCC1 / XPF complex makes the 5' incision. ERCC1 / XPF probably plays a role in processing partially processed recombination intermediates.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DNA excision repair protein ERCC-4; DNA repair endonuclease subunit; DNA repair endonuclease XPF; DNA repair protein complementing XP-F cells; Ercc4 protein-like; excision repair cross-complementation group 4; excision repair cross-complementing rodent repair deficiency, complementation group 4; excision-repair, complementing defective, in Chinese hamster; Xeroderma pigmentosum group F-complementing protein; xeroderma pigmentosum, complementation group F
基因别名: AI606920; ERCC11; ERCC4; FANCQ; RAD1; RGD1560340; XFEPS; XPF
UniProt ID: (Human) Q92889, (Mouse) Q9QZD4
Entrez Gene ID: (Human) 2072, (Mouse) 50505, (Rat) 304719
single-stranded DNA endodeoxyribonuclease activity
TFIID-class transcription factor binding
damaged DNA binding
single-stranded DNA binding
endodeoxyribonuclease activity
protein binding
protein C-terminus binding
structure-specific DNA binding
protein N-terminus binding
nucleic acid binding
DNA binding
nuclease activity
endonuclease activity
hydrolase activity
phosphoric ester hydrolase activity
T/G mismatch-specific endonuclease activity
retroviral 3' processing activity
DNA metabolism protein
endodeoxyribonuclease
nucleic acid metabolism protein
resolution of meiotic recombination intermediates
telomere maintenance
double-strand break repair via homologous recombination
DNA repair
transcription-coupled nucleotide-excision repair
nucleotide-excision repair
nucleotide-excision repair, preincision complex stabilization
nucleotide-excision repair, DNA incision, 3'-to lesion
nucleotide-excision repair, DNA incision, 5'-to lesion
response to UV
UV protection
negative regulation of telomere maintenance
nucleotide-excision repair, DNA incision
cellular response to UV
interstrand cross-link repair
global genome nucleotide-excision repair
nucleotide-excision repair involved in interstrand cross-link repair
DNA metabolic process
cellular response to DNA damage stimulus
DNA catabolic process, endonucleolytic
obsolete telomere maintenance via telomere shortening
