Invitrogen

ZO-1 Polyclonal Antibody

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ZO-1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ZO-1 (red) in Caco-2 cells. The cells were blocked with 3% BSA in PBS (Product # 37525) for 15 minutes at room temperature, stained with a ZO-1 Rabbit polyclonal antibody (Product # 18-7430) at a concentration of 1:500 in blocking buffer for at least 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 647 conjugate (Product # A27040) at a dilution of 1:1000 for 30 minutes at room temperature (red). Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

ZO-1 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Figure 2 Itgb1 CKO/- ; Shh Cre/+ mutant lung epithelial cells reorient mitotic spindles and show partial loss of apical-basal polarity. (A) Confocal sections of whole-mount E11 lungs immunostained for markers of mitosis (phospho-Histone 3, pH3), mitotic spindles (acetylated Tubulin, AcTUB) and the epithelium (E-Cadherin, ECAD). Representative images are shown for mitotic cells with no obvious mitotic spindle (prophase) and with the mitotic spindle orientated between 0 to 45 degrees (0deg~45deg) or 45 to 90 degrees (45deg~90deg) relative to the lumenal surface of the epithelium. Mitotic cells in the control lung are exclusively localized within one nuclear diameter of the lumenal surface of the epithelium. The Itgb1 CKO/- ; Shh Cre/+ mutant lung contains mitotic cells localized away from the lumenal surface and sometimes on the basal surface of the epithelium. Grey scale images of the ECAD and AcTUB staining are shown below for the mitotic cells of interest. Note the intense ECAD puncta associated with the basally located Itgb1 mutant nuclei. The upper and lower dashed lines demarcate the lumenal (Lumen) and basal side of the epithelium, respectively. Scale bar, 10 um. (B) Quantification of the percentage of cells in each category in ( A ) out of 110 (control) and 127 (mutant) mitotic cells from 3 E11 lungs. The filled columns and the associated numbers indicate the percentage of mitotic cells that are located more than one nuclear diameter from the lumenal surface of the epit

ZO-1 Antibody in Immunohistochemistry (IHC) — Fig. 8 High-magnification images comparing claudin localization with ZO-1 and occludin: photomicrographs showing serial sections of human renal cortical tissue stained for claudin-14 ( A ) and ZO-1 ( B ) with a second sequence of serial sections showing staining of claudin-3 ( C ), -4 ( D ) and -7 ( E ) with occludin ( F ) [claudin-14 was seen in basolateral locations in tubules corresponding to those with discrete tight junction staining for ZO-1 (A and B, square symbol marks corresponding tubule); in serial sections from the same tissue sample, claudin-4 was observed to be mostly in discrete tight junction staining patterns (D), whereas claudin-3 and -7 showed strong basolateral cell membrane staining in addition to tight junction staining (C and E, asterisks mark corresponding tubule); in corresponding tubules, occludin was stained in a discrete pattern consistent with tight junction localization (F); scale bar 50 um].

产品信息

18-7430

应用	建议稀释比	已发表文章
免疫组化（石蜡） (IHC (P))	Assay-dependent	-
免疫细胞化学 (ICC/IF)	1:250-1:1,000	查看6篇已发表文章 6篇已发表文章
流式细胞分析 (Flow)	-	查看1篇已发表文章 1篇已发表文章
免疫组织化学（多聚甲醛固定） (IHC (PFA))	-	查看1篇已发表文章 1篇已发表文章
免疫组化 (IHC)	-	查看3篇已发表文章 3篇已发表文章
免疫组化（冰冻） (IHC (F))	-	查看1篇已发表文章 1篇已发表文章

产品规格
种属反应	Human
已发表种属	Human, Mouse, Rat
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Peptide sequence from the N-terminal region of human ZO-1. 查看免疫原
偶联物	Unconjugated
形式	Liquid
纯化类型	purified
保存液	PBS, pH 7.4, with 1% BSA
内含物	0.1% sodium azide
保存条件	4° C
RRID	AB_2533048

靶标信息

ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Transplantation of GMP-grade human iPSC-derived retinal pigment epithelial cells in rodent model: the first pre-clinical study for safety and efficacy in China.

Annals of translational medicine

Zhang H,Su B,Jiao L,Xu ZH,Zhang CJ,Nie J,Gao ML,Zhang YV,Jin ZB

18-7430 was used in Immunohistochemistry (PFA fixed), Flow Cytometry to provide further evidence for the use of human iRPE transplantation to treat retinal degenerative disease in pre-clinical animal models.

Mon Feb 01 00:00:00 EST 2021

Transplantation of GMP-grade human iPSC-derived retinal pigment epithelial cells in rodent model: the first pre-clinical study for safety and efficacy in China.

Annals of translational medicine

Zhang H,Su B,Jiao L,Xu ZH,Zhang CJ,Nie J,Gao ML,Zhang YV,Jin ZB

Mon Feb 01 00:00:00 EST 2021

Inhibition of TGF-β1 Signaling by IL-15: A Novel Role for IL-15 in the Control of Renal Epithelial-Mesenchymal Transition: IL-15 Counteracts TGF-β1-Induced EMT in Renal Fibrosis.

International journal of cell biology

Devocelle A,Lecru L,François H,Desterke C,Gallerne C,Eid P,Estelle O,Azzarone B,Giron-Michel J

Published figure using ZO-1 polyclonal antibody (Product # 18-7430) in Immunocytochemistry

Wed Sep 30 00:00:00 EDT 2020

Development of a Scalable Coculture System for Gut Anaerobes and Human Colon Epithelium.

Gastroenterology

Sasaki N,Miyamoto K,Maslowski KM,Ohno H,Kanai T,Sato T

18-7430 was used in Immunocytochemistry to establish iHACS, a system that provides optimal growth environments for human colonic epithelium and gut anaerobes.

Wed Jul 01 00:00:00 EDT 2020

A C-terminal peptide from type I interferon protects the retina in a mouse model of autoimmune uveitis.

PloS one

Ahmed CM,Ildefonso CJ,Johnson HM,Lewin AS

18-7430 was used in Immunohistochemistry to propose that IFNα-C peptide exhibits anti-inflammatory properties and protects autoimmune uveitis mice against damage to retinal structure and function.

Wed Apr 22 00:00:00 EDT 2020

Elovanoids are novel cell-specific lipid mediators necessary for neuroprotective signaling for photoreceptor cell integrity.

Scientific reports

Jun B,Mukherjee PK,Asatryan A,Kautzmann MA,Heap J,Gordon WC,Bhattacharjee S,Yang R,Petasis NA,Bazan NG

Published figure using ZO-1 polyclonal antibody (Product # 18-7430) in Immunofluorescence

Thu Jul 13 00:00:00 EDT 2017

Directing Differentiation of Pluripotent Stem Cells Toward Retinal Pigment Epithelium Lineage.

Stem cells translational medicine

Choudhary P,Booth H,Gutteridge A,Surmacz B,Louca I,Steer J,Kerby J,Whiting PJ

Published figure using ZO-1 polyclonal antibody (Product # 18-7430) in Immunocytochemistry

Wed Feb 01 00:00:00 EST 2017

Targeting the cAMP and Transforming Growth Factor-β Pathway Increases Proliferation to Promote Re-Epithelialization of Human Stem Cell-Derived Retinal Pigment Epithelium.

Stem cells translational medicine

Choudhary P,Gutteridge A,Impey E,Storer RI,Owen RM,Whiting PJ,Bictash M,Benn CL

Published figure using ZO-1 polyclonal antibody (Product # 18-7430) in Immunocytochemistry

Fri Jul 01 00:00:00 EDT 2016

A FOXM1 Dependent Mesenchymal-Epithelial Transition in Retinal Pigment Epithelium Cells.

PloS one

Choudhary P,Dodsworth BT,Sidders B,Gutteridge A,Michaelides C,Duckworth JK,Whiting PJ,Benn CL

18-7430 was used in immunocytochemistry to determine the molecular mechanisms that affect the epithelial phenotype.

Tue Mar 29 00:00:00 EDT 2016

Lung epithelial branching program antagonizes alveolar differentiation.

Proceedings of the National Academy of Sciences of the United States of America

Chang DR,Martinez Alanis D,Miller RK,Ji H,Akiyama H,McCrea PD,Chen J

18-7430 was used in Immunohistochemistry to show that an epithelial branching morphogenesis program antagonises alveolar differentiation in the mouse lung.

Tue Nov 05 00:00:00 EST 2013

Integrin Beta 1 suppresses multilayering of a simple epithelium.

PloS one

Chen J,Krasnow MA

Published figure using ZO-1 polyclonal antibody (Product # 18-7430) in Immunofluorescence

Tue Jul 23 00:00:00 EDT 2013

Differential expression of claudin tight junction proteins in the human cortical nephron.

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association

Kirk A,Campbell S,Bass P,Mason J,Collins J

Published figure using ZO-1 polyclonal antibody (Product # 18-7430) in Immunohistochemistry

Thu Jul 01 00:00:00 EDT 2010

生物信息学

蛋白别名: DKFZp686M05161; MGC133289; OTTHUMP00000174520; Tight junction protein 1; Tight junction protein ZO-1; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1

基因别名: TJP1; ZO-1; ZO1

UniProt ID: (Human) B4E3K1

Entrez Gene ID: (Human) 7082

分子生物学功能: tight junction

功能

blastocyst formation cell-cell junction assembly sensory perception of sound hippo signaling establishment of endothelial intestinal barrier cell-cell adhesion cell-cell signaling involved in cell-cell junction organization

参与通路

protein binding calmodulin binding protein domain specific binding cadherin binding involved in cell-cell adhesion

ZO-1 Polyclonal Antibody

产品信息

18-7430

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

免疫组织化学（多聚甲醛固定） (IHC (PFA))

免疫组化 (IHC)

免疫组化（冰冻） (IHC (F))

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

纯化类型

保存液

内含物

保存条件

RRID

靶标信息

生物信息学

性能保障

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