Invitrogen

ZO-3 Polyclonal Antibody

ZO-3 Antibody in Immunocytochemistry (ICC/IF)

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ZO-3 Antibody (36-4000) in ICC/IF

Immunofluorescent analysis of ZO-3/TJP3 Antibody was done on 90% confluent log phase CACO2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ZO-3/TJP3 Antibody (Product # 36-4000) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 45 minutes at ro... View More

ZO-3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

36-4000

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

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免疫组化（石蜡） (IHC (P))

1:20-1:200

免疫细胞化学 (ICC/IF)

1 µg/mL

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酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫沉淀 (IP)

Assay-dependent

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其他PubMed (Misc)

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产品规格

种属反应

Dog, Human, Mouse, Rat

已发表种属

Dog, Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide derived from an internal region of the human ZO-3 protein.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533256

产品详细信息

36-4000 was used successfully in the western blot analysis of ZO-3 in MDCK cell lysate.

靶标信息

ZO-3 is a member of the membrane associated guanylate kinse (MAGUK) family and are considered tight junction-associated proteins. Tight junctions are complexes of proteins that create intercellular boundaries between the plasma membrane domains of epithelial and endothelial cells. ZO-3 is believed to have both structural and signaling roles, and is characteristically defined by three protein-protein interaction modules: the PDZ domain, the SH3 domain and the guanylate kinase (GuK) domain. ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

E-cadherin is downregulated in benign prostatic hyperplasia and required for tight junction formation and permeability barrier in the prostatic epithelial cell monolayer.

The Prostate

Li F,Pascal LE,Stolz DB,Wang K,Zhou Y,Chen W,Xu Y,Chen Y,Dhir R,Parwani AV,Nelson JB,DeFranco DB,Yoshimura N,Balasubramani GK,Gingrich JR,Maranchie JK,Jacobs BL,Davies BJ,Hrebinko RL,Bigley JD,McBride D,Guo P,He D,Wang Z

36-4000 was used in Western Blotting to indicate that tight junctions are compromised in BPH and loss of E-cadherin is potentially an important underlying mechanism, suggesting targeting E-cadherin loss could be a potential approach to prevent or treat BPH.

Thu Aug 01 00:00:00 EDT 2019

Delineation of the key aspects in the regulation of epithelial monolayer formation.

Molecular and cellular biology

Aschauer L,Gruber LN,Pfaller W,Limonciel A,Athersuch TJ,Cavill R,Khan A,Gstraunthaler G,Grillari J,Grillari R,Hewitt P,Leonard MO,Wilmes A,Jennings P

Mon Jul 01 00:00:00 EDT 2013

Delineation of the key aspects in the regulation of epithelial monolayer formation.

Molecular and cellular biology

Aschauer L,Gruber LN,Pfaller W,Limonciel A,Athersuch TJ,Cavill R,Khan A,Gstraunthaler G,Grillari J,Grillari R,Hewitt P,Leonard MO,Wilmes A,Jennings P

Mon Jul 01 00:00:00 EDT 2013

Family-wide investigation of PDZ domain-mediated protein-protein interactions implicates β-catenin in maintaining the integrity of tight junctions.

Chemistry & biology

Gujral TS,Karp ES,Chan M,Chang BH,MacBeath G

Published figure using ZO-3 polyclonal antibody (Product # 36-4000) in Immunofluorescence

Thu Jun 20 00:00:00 EDT 2013

Family-wide investigation of PDZ domain-mediated protein-protein interactions implicates β-catenin in maintaining the integrity of tight junctions.

Chemistry & biology

Gujral TS,Karp ES,Chan M,Chang BH,MacBeath G

Published figure using ZO-3 polyclonal antibody (Product # 36-4000) in Immunofluorescence

Thu Jun 20 00:00:00 EDT 2013

Macrophages and dendritic cells express tight junction proteins and exchange particles in an in vitro model of the human airway wall.

Immunobiology

Blank F,Wehrli M,Lehmann A,Baum O,Gehr P,von Garnier C,Rothen-Rutishauser BM

36-4000 was used in immunocytochemistry to study barrier integrity and tight junction protein expression using in vitro lung epithelial barrier model

Mon Jul 18 00:00:00 EDT 2011

Connexin47, connexin29 and connexin32 co-expression in oligodendrocytes and Cx47 association with zonula occludens-1 (ZO-1) in mouse brain.

Neuroscience

Li X,Ionescu AV,Lynn BD,Lu S,Kamasawa N,Morita M,Davidson KG,Yasumura T,Rash JE,Nagy JI

Tue Sep 14 00:00:00 EDT 2004

Connexin47, connexin29 and connexin32 co-expression in oligodendrocytes and Cx47 association with zonula occludens-1 (ZO-1) in mouse brain.

Neuroscience

Li X,Ionescu AV,Lynn BD,Lu S,Kamasawa N,Morita M,Davidson KG,Yasumura T,Rash JE,Nagy JI

Tue Sep 14 00:00:00 EDT 2004

Connexin47, connexin29 and connexin32 co-expression in oligodendrocytes and Cx47 association with zonula occludens-1 (ZO-1) in mouse brain.

Neuroscience

Li X,Ionescu AV,Lynn BD,Lu S,Kamasawa N,Morita M,Davidson KG,Yasumura T,Rash JE,Nagy JI

Tue Sep 14 00:00:00 EDT 2004

Connexin47, connexin29 and connexin32 co-expression in oligodendrocytes and Cx47 association with zonula occludens-1 (ZO-1) in mouse brain.

Neuroscience

Li X,Ionescu AV,Lynn BD,Lu S,Kamasawa N,Morita M,Davidson KG,Yasumura T,Rash JE,Nagy JI

Tue Sep 14 00:00:00 EDT 2004

生物信息学

蛋白别名: Tight junction protein 3; tight junction protein 3 (zona occludens 3); Tight junction protein ZO-3; TJP-3; Zona occludens protein 3; Zonula occludens protein 3

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基因别名: TJP3; ZO-3; ZO3

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UniProt ID: (Human) O95049

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Entrez Gene ID: (Human) 27134, (Dog) 403852, (Mouse) 27375, (Rat) 314640

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功能

protein binding tight junction

参与通路

regulation of G1/S transition of mitotic cell cycle