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Invitrogen
ZRANB3 Polyclonal Antibody
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ZRANB3 Antibody (PA5-97227) in WB
Western blot analysis of ZRANB3 in extracts of various cell lines. Samples were incubated with ZRANB3 Polyclonal antibody (Product # PA5-97227) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
产品信息
PA5-97227
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein (or fragment)
偶联物
形式
Liquid
浓度
1.14 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: ITKQQTKQNC TKRYITKEDV AVASMDKVKN VGGHVRLITK ESRPRDPFTK KLLEDGACVP FLNPYTVQAD LTVKPSTSKG YLQAVDNEGN PLCLRCQQPT CQTKQACKAN SWDSRFCSLK CQEEFWIRSN NSYLRAKVFE TEHGVCQLCN VNAQELFLRL RDAPKSQRKN LLYATWTSKL PLEQLNEMIR NPGEGHFWQV DHIKPVYGGG GQCSLDNLQT LCTVCHKERT ARQAKERSQV RRQSLASKHG SDITRFLVKK; Positive Samples: HeLa, Raji, Jurkat, 293T, Mouse liver, Mouse heart, Mouse brain, Rat liver; Cellular Location: Chromosome, Nucleus
靶标信息
DNA annealing helicase and endonuclease required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events (PubMed:21078962, PubMed:22704558, PubMed:22705370, PubMed:22759634, PubMed:26884333). Recruited to the sites of stalled DNA replication by polyubiquitinated PCNA and acts as a structure-specific endonuclease that cleaves the replication fork D-loop intermediate, generating an accessible 3'-OH group in the template of the leading strand, which is amenable to extension by DNA polymerase (PubMed:22759634). In addition to endonuclease activity, also catalyzes the fork regression via annealing helicase activity in order to prevent disintegration of the replication fork and the formation of double-strand breaks (PubMed:22705370, PubMed:22704558). [UniProt]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: AH2; Annealing helicase 2; DNA annealing helicase and endonuclease ZRANB3; Zinc finger Ran-binding domain-containing protein 3; zinc finger, RAN-binding domain containing 3
基因别名: 4933425L19Rik; AH2; AI316834; C730006D09; RGD1564952; ZRANB3
UniProt ID: (Human) Q5FWF4, (Mouse) Q6NZP1
Entrez Gene ID: (Human) 84083, (Rat) 304761, (Mouse) 226409
DNA helicase activity
endodeoxyribonuclease activity
protein binding
ATP binding
zinc ion binding
annealing helicase activity
K63-linked polyubiquitin binding
DNA binding
nucleotide binding
nucleic acid binding
catalytic activity
helicase activity
nuclease activity
endonuclease activity
hydrolase activity
phosphoric ester hydrolase activity
T/G mismatch-specific endonuclease activity
retroviral 3' processing activity
metal ion binding
DNA metabolism protein
DNA strand renaturation
DNA repair
cellular response to DNA damage stimulus
response to UV
replication fork processing
DNA rewinding
negative regulation of DNA recombination
replication fork protection
nucleic acid phosphodiester bond hydrolysis
DNA catabolic process, endonucleolytic
metabolic process
