MUbio

actin beta-cytoplasmic Monoclonal Antibody (4C2)

Name: actin beta-cytoplasmic Monoclonal Antibody (4C2)
Brand: MUbio
SKU: MUB0110P

查看其他59个beta Actin抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

产品信息

MUB0110P

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:5,000

免疫组化（石蜡） (IHC (P))

1:100-1:500

免疫组化（冰冻） (IHC (F))

1:100-1:500

流式细胞分析 (Flow)

Assay-dependent

酶联免疫吸附实验 (ELISA)

Assay-dependent

产品规格

种属反应

Chicken, Human, Mouse, Pig, Rabbit, Rat, Zebrafish

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

4C2

抗原

actin beta-cytoplasmic

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A/G

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

4C2 is a mouse monoclonal Ig1 antibody highly specific for beta-cytoplasmic actin.

The epitope recognized by 4C2 is highly conserved. The antibody therefore cross-reacts with many species including protochordates, lower craniates and mammals.

The antibody may be stored at 4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

靶标信息

Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: actin, beta; Actin, cytoplasmic 1; B-ACTZF; Bact; actin; beta actin; beta cytoskeletal actin; Beta-actin; Beta-actin-1; cytoplasmic 1; cytoplasmic beta-actin; cytoskeletal beta actin; gamma non-muscle actin; PS1TP5-binding protein 1

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基因别名: ACT-5; ACTB; actba; actin; Actx; B-ACTZF; Bact; bactin1; bactzf; beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1; wu:fd18f05