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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • alpha Tubulin Antibodies

          Rockland

          alpha-Tubulin Monoclonal Antibody (17H11.F10)

          查看其他60个alpha Tubulin抗体
          说明书
          实验方案
          问题与答案
          技术支持
          说明书
          实验方案
          问题与答案
          技术支持

          Cite alpha-Tubulin Monoclonal Antibody (17H11.F10)

          • 抗体检测数据 (13)
          alpha-Tubulin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          alpha-Tubulin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          图: 1 / 13

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          alpha-Tubulin Antibody (200-301-880) in ICC/IF

          Immunofluorescence microscopy of Anti-tubulin in U-87 MG cells using FITC-conjugated Fluorescent TrueBlot® anti-mouse IgG (p/n 18-0217-32) for detection. U87-MG cells were fixed with 100% methanol, permeabilized with 0.3% Triton X-100, blocked with 5% rat serum/0.3 Triton X-100 for 1 hr, then incubated with 15 µg/mL of anti-a-tubulin primary antibody (p/n 200-301-880) at 4°C overnight. Following 3 washes in... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
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          alpha-Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha-Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha-Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha-Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha-Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha-Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha-Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha-Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha-Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha-Tubulin Antibody in Immunocytochemistry (ICC/IF)
          alpha-Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          alpha-Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          alpha-Tubulin Antibody in Western Blot (WB)

          产品信息

          200-301-880

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:1,000
          -

          免疫组化(石蜡) (IHC (P))

          2.5 µg/mL
          -

          免疫细胞化学 (ICC/IF)

          0.1 µg/mL
          -

          酶联免疫吸附实验 (ELISA)

          1:300,000
          -

          免疫沉淀 (IP)

          1:1,000
          -
          产品规格

          种属反应

          Bovine, Chicken, Human, Mouse, Rat

          宿主/亚型

          Mouse / IgG1, kappa

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          17H11.F10

          抗原

          Anti-Tubulin Loading Control Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of human alpha tubulin protein.
          查看免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          1 mg/mL

          纯化类型

          Protein A

          保存液

          0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

          内含物

          0.01% sodium azide

          保存条件

          -20°C, Avoid Freeze/Thaw Cycles

          运输条件

          Ambient (domestic); Wet ice (international)

          产品详细信息

          Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

          This Loading Control antibody is directed against alpha tubulin. A BLAST analysis was used to suggest antibody reactivity with alpha tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.

          靶标信息

          Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          篇参考文献 (0)

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          生物信息学

          蛋白别名: alpha 4 tubulin; alpha tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3E; Alpha-tubulin 4; Alpha-tubulin 6; Alpha-tubulin isotype M-alpha-1; Alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; Alpha-tubulin isotype M-alpha-6; Alpha-tubulin ubiquitous; Beta-tubulin class-I; Beta-tubulin class-II; beta-tubulin T beta15 (aa 1-445); class II beta-tubulin isotype; class IIb beta-tubulin; FLJ30169; H2 ALPHA; hum-a-tub1; hum-a-tub2; T beta-15; Talpha1 alpha-tubulin; Testis-specific; Testis-specific alpha-tubulin; TUBA1/3/4; TUBB; Tubulin alpha; tubulin alpha 1c; tubulin alpha 6; Tubulin alpha chain-like 3; Tubulin alpha-1 chain; Tubulin alpha-1A chain; Tubulin alpha-1B chain; Tubulin alpha-1C chain; Tubulin alpha-2 chain; Tubulin alpha-3 chain; Tubulin alpha-3C chain; Tubulin alpha-3E chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin alpha-5 chain; Tubulin alpha-6 chain; tubulin alpha-8 chain; Tubulin alpha-ubiquitous chain; Tubulin B-alpha-1; Tubulin beta-1 chain; Tubulin beta-2 chain; Tubulin beta-2B chain; Tubulin H2-alpha; Tubulin K-alpha-1; tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 1a; tubulin, alpha 1b; tubulin, alpha 1c; tubulin, alpha 2; tubulin, alpha 3; tubulin, alpha 3c; tubulin, alpha 3e; tubulin, alpha 4; tubulin, alpha 4a; tubulin, alpha 6; tubulin, alpha 8; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; tubulin, alpha-like 3; tubulin, beta 2; tubulin, beta 2B class IIb; tubulin, beta polypeptide; tubulin, beta polypeptide paralog; unnamed protein product

          查看更多 收起

          基因别名: 2410129E14Rik; ALS22; B-ALPHA-1; bA408E5.3; bA506K6.1; bcm948; brdp; H2-ALPHA; K-ALPHA-1; LIS3; M[a]4; M[a]6; PMGYSA; RGD1309427; RGD1565476; Tuba-1; TUBA1; TUBA1A; TUBA1B; TUBA1C; TUBA2; TUBA3; TUBA3C; TUBA3D; TUBA3E; Tuba4; TUBA4A; TUBA6; TUBA8; TUBAL3; TUBAT; TUBB2; Tubb2a; TUBB2B

          查看更多 收起

          UniProt ID: (Human) Q71U36, (Bovine) P81947, (Human) P68363, (Bovine) Q3ZCJ7, (Human) Q9BQE3, (Human) Q6PEY3, (Human) Q6PEY2, (Bovine) Q32KN8, (Human) P68366, (Bovine) P81948, (Chicken) P08070, (Human) A6NHL2, (Bovine) Q6B856, (Human) A8K068, (Chicken) P32882, (Mouse) P68369, (Rat) P68370, (Mouse) P05213, (Rat) Q6P9V9, (Mouse) P68373, (Rat) Q6AYZ1, (Mouse) P68368, (Rat) Q5XIF6, (Mouse) Q3UX10, (Mouse) Q9CWF2, (Rat) Q3KRE8

          查看更多 收起

          Entrez Gene ID: (Chicken) 100857858, (Human) 7846, (Bovine) 404053, (Chicken) 429035, (Bovine) 539882, (Human) 10376, (Bovine) 504244, (Human) 84790, (Human) 7278, (Human) 112714, (Bovine) 534900, (Chicken) 100857247, (Human) 7277, (Bovine) 777775, (Chicken) 421169, (Chicken) 418166, (Chicken) 396426, (Human) 79861, (Chicken) 416694, (Bovine) 538631, (Bovine) 281555, (Human) 347733, (Chicken) 420883, (Mouse) 22142, (Rat) 64158, (Mouse) 22143, (Rat) 500929, (Mouse) 22146, (Rat) 300218, (Mouse) 22145, (Rat) 316531, (Rat) 291287, (Mouse) 238463, (Mouse) 73710, (Rat) 291081

          查看更多 收起

          功能
          GTPase activity structural molecule activity structural constituent of cytoskeleton protein binding GTP binding protein domain specific binding double-stranded RNA binding ubiquitin protein ligase binding molecular_function enzyme binding protein kinase binding protein heterodimerization activity calcium ion binding nucleotide binding tubulin
          参与通路
          G2/M transition of mitotic cell cycle cytoskeleton organization microtubule-based process cytoskeleton-dependent intracellular transport cell division microtubule cytoskeleton organization mitotic cell cycle cellular response to interleukin-4 biological_process platelet degranulation positive regulation of axon guidance neuron migration negative regulation of microtubule polymerization protein polymerization metabolic process
          It has to be done as per old AB suggested Products section.
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          只要您发现Invitrogen抗体在实验中的表现与网站或说明书的所述内容不符,您即可享受免费退换货服务。*

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