Invitrogen

beta-2 Microglobulin Polyclonal Antibody

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beta-2 Microglobulin Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of beta-2 Microglobulin in A431 cell. Antigen retrieval was performed on the tissue using IHC enzyme antigen retrieval reagent and blocked with 10% goat serum. Samples were incubated with beta-2 Microglobulin polyclonal antibody (Product # PA5-95426) at a 2 µg/1x10^6 cells dilution, followed by 488 conjugated goat anti-rabbit IgG using a 1:100 dilution and DAPI.

beta-2 Microglobulin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of beta-2 Microglobulin in paraffin-embedded human intestinal cancer tissues. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with beta-2 Microglobulin polyclonal antibody (Product # PA5-95426) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.

beta-2 Microglobulin Antibody in Western Blot (WB) — Western blot analysis of beta-2 Microglobulin in Lane 1: HEPG2 whole cell lysates, Lane 2: 22RV1 whole cell lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with beta-2 Microglobulin polyclonal antibody (Product # PA5-95426) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).

beta-2 Microglobulin Antibody in Flow Cytometry (Flow) — Flow cytometry of beta-2 Microglobulin in U251 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with beta-2 Microglobulin polyclonal antibody (Product # PA5-95426) at a dilution of 1 µg (per 1x10^6 cells), followed by 488 conjugated goat anti-rabbit IgG (30 min at 20°C) using a 5-10 µg (per 1x10^6 cells) dilution.

产品细节

Applications

Tested Dilution

Publications

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫组化（冰冻） (IHC (F))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human Beta 2 Microglobulin recombinant protein (Position: Q22-M119).

偶联物

Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2807229

产品详细信息

Human Beta 2 Microglobulin shares 69.4% and 74.5% amino acid (aa) sequence identity with mouse and rat Beta 2 Microglobulin, respectively.

Reconstitution information: Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

靶标信息

Beta-2-microglobulin (B2M) is a 12-14 kDa protein that is a subunit of the major histocompatibility complex (MHC) class I. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells, but is absent on erythrocytes. Beta-2 microglobulin is also found in free form in the plasma. The amino acid sequence of Beta-2-microglobulin is homologous to the constant regions of immunoglobulin heavy and light chains, with a predominantly beta pleated sheet structure. The protein is ubiquitously expressed in the cytoplasm, and is involved in antigen processing and presentation. Beta-2-microglobulin also plays a role in TCR signaling, and downstream signaling in naive CD8+ cells. Beta 2-microglobulin is encoded by the B2M gene located on chromosome 15. A mutation in the Beta-2-microglobulin gene has been shown to result in hypercatabolic hypoproteinemia. Levels of beta 2 microglobulin can be used to assess renal function in kidney transplant recipients. Beta-2-microglobulin that protein can be used as a prognostic marker for HIV infection.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: B-F; B2-microglobulin; beta chain of MHC class I molecules; beta-2-microglobin; Beta-2-microglobulin; Beta-2-microglobulin form pI 5.3; Beta2 microglobulin; MHC Class I; microglobulin beta-2; Orla-B2m

基因别名: B2M; CDABP0092; HDCMA22P; IMD43

UniProt ID: (Human) P61769

Entrez Gene ID: (Human) 567

分子生物学功能: major histocompatibility complex protein