Invitrogen

beta-2 Microglobulin Polyclonal Antibody

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beta-2 Microglobulin Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of Beta 2 Microglobulin using anti- Beta 2 Microglobulin antibody (Product # PA5-95426) . Beta 2 Microglobulin was detected in a section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti- Beta 2 Microglobulin antibody (Product # PA5-95426) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

beta-2 Microglobulin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Beta 2 Microglobulin in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti- Beta 2 Microglobulin antibody (Product # PA5-95426) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

beta-2 Microglobulin Antibody in Western Blot (WB) — Western blot analysis of Beta 2 Microglobulin in, Lane 1: HEPG2 whole Cell lysates, Lane 2: 22RV1 whole cell lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. The membrane was blocked with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with beta-2 Microglobulin Polyclonal Antibody (Product # PA5-95426) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0. 1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit. A specific band was detected for Beta 2 Microglobulin at approximately 13KD. The expected band size for Beta 2 Microglobulin is at 13KD.

beta-2 Microglobulin Antibody in Flow Cytometry (Flow) — Flow Cytometry of beta-2 Microglobulin in U251 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with beta-2 Microglobulin Polyclonal Antibody (Product # PA5-95426) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.

产品信息

PA5-95426

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human Beta 2 Microglobulin recombinant protein (Position: Q22-M119).

查看免疫原

偶联物

Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2807229

产品详细信息

Human Beta 2 Microglobulin shares 69.4% and 74.5% amino acid (aa) sequence identity with mouse and rat Beta 2 Microglobulin, respectively.

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

Beta-2-microglobulin (B2M) is a 12-14 kDa protein that is a subunit of the major histocompatibility complex (MHC) class I. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells, but is absent on erythrocytes. Beta-2 microglobulin is also found in free form in the plasma. The amino acid sequence of Beta-2-microglobulin is homologous to the constant regions of immunoglobulin heavy and light chains, with a predominantly beta pleated sheet structure. The protein is ubiquitously expressed in the cytoplasm, and is involved in antigen processing and presentation. Beta-2-microglobulin also plays a role in TCR signaling, and downstream signaling in naive CD8+ cells. Beta 2-microglobulin is encoded by the B2M gene located on chromosome 15. A mutation in the Beta-2-microglobulin gene has been shown to result in hypercatabolic hypoproteinemia. Levels of beta 2 microglobulin can be used to assess renal function in kidney transplant recipients. Beta-2-microglobulin that protein can be used as a prognostic marker for HIV infection.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: B-F; B2-microglobulin; Beta 2 microglobin; Beta 2 microglobulin; beta chain of MHC class I molecules; beta-2-microglobin; Beta-2-microglobulin; Beta-2-microglobulin form pI 5.3; Beta2 microglobulin; MHC Class I; microglobulin beta-2; Orla-B2m

基因别名: B2M; CDABP0092; HDCMA22P; IMD43

UniProt ID: (Human) P61769

Entrez Gene ID: (Human) 567

分子生物学功能: major histocompatibility complex protein

功能

参与通路

glycoprotein binding protein binding identical protein binding