Invitrogen

beta-4 Tubulin Monoclonal Antibody (OTI3F1)

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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说明书

实验方案

技术支持

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beta-4 Tubulin Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of TUBB4 in COS7 cells. Cells were transfected with a plasmid overexpressing TUBB4 and probed with a TUBB4 monoclonal antibody (Product # MA5-25561).

beta-4 Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human ovary tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a TUBB4 monoclonal antibody (Product # MA5-25561).

beta-4 Tubulin Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of SK-N-AS (Lane 1), SH-SY5Y (Lane 2), HEL 92.1.7 (Lane 3), A549 (Lane 4), tissue extracts of Mouse Brain (Lane 5), Rat Brain (Lane 6) and Mouse Skin (Lane 7). The blot was probed with Anti- beta-4 Tubulin Monoclonal Antibody (OTI3F1) (Product # MA5-25561, 1:500 dilution) and detected by chemiluminescence using Goat anti Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 51 kDa band corresponding to beta-4 Tubulin was detected across cell lines and tissues tested except Mouse Skin which is reported to be negative for beta-4 Tubulin expression.

beta-4 Tubulin Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines and tissues owing to their inherent genetic constitution. Relative expression of beta-4 Tubulin was observed in all the cell lines and tissues tested except for Mouse Skin using Anti- beta-4 Tubulin Monoclonal Antibody (OTI3F1) (Product # MA5-25561) in Western Blot. {RE}

产品信息

MA5-25561

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:500-1:2,000	-
免疫组化（石蜡） (IHC (P))	1:150	-
免疫细胞化学 (ICC/IF)	1:100	-
流式细胞分析 (Flow)	1:100	-

产品规格
种属反应	Dog, Human, Mouse, Non-human primate, Rat
宿主/亚型	Mouse / IgG2a
分类	Monoclonal
类型	Antibody
克隆号	OTI3F1
抗原	Full length human recombinant protein of TUBB4 produced in HEK293T cell
偶联物	Unconjugated
形式	Liquid
浓度	1.0 mg/mL
纯化类型	Affinity Chromatography
保存液	PBS, pH 7.3, with 1% BSA, 50% glycerol
内含物	0.02% sodium azide
保存条件	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2722896

靶标信息

TUBB4A (beta-4 tubulin) encodes a member of the beta tubulin family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Mutations in this gene cause hypomyelinating leukodystrophy-6 and autosomal dominant torsion dystonia-4. Alternate splicing results in multiple transcript variants encoding different isoforms. A pseudogene of this gene is found on chromosome X.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: class IVa beta-tubulin; dystonia 4, torsion (autosomal dominant); Tubulin 5 beta; tubulin beta; Tubulin beta-4 chain; Tubulin beta-4A chain; tubulin, beta 4; tubulin, beta 4 class IVa; tubulin, beta 4A class IVa; tubulin, beta, 5

基因别名: AI325297; beta-5; DYT4; M(beta)4; Tubb; TUBB4; TUBB4A; TUBB5

UniProt ID: (Human) P04350, (Mouse) Q9D6F9

Entrez Gene ID: (Dog) 100855815, (Human) 10382, (Mouse) 22153, (Rat) 29213

分子生物学功能: tubulin