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| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫印迹 (WB) |
1:5,000-1:20,000 | - |
免疫组化 (IHC) |
Assay-dependent | - |
免疫组化（石蜡） (IHC (P)) |
1:100-1:1,000 | - |
免疫细胞化学 (ICC/IF) |
1:100-1:1,000 | - |
免疫沉淀 (IP) |
1:100-1:1,000 | - |
| 产品规格 | |
|---|---|
种属反应 |
Fruit fly, Human, Mouse, Rat |
宿主/亚型 |
Rabbit / IgG |
分类 |
Polyclonal |
类型 |
Antibody |
抗原 |
Recombinant protein encompassing a sequence within the center region of human beta Actin. |
偶联物 |
Unconjugated |
形式 |
Liquid |
浓度 |
0.19 mg/mL |
纯化类型 |
Antigen affinity chromatography |
保存液 |
PBS, pH 7, with 20% glycerol, 1% BSA |
内含物 |
0.025% ProClin 300 |
保存条件 |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2792434 |
Keep as concentrated solution.
Predicted reactivity: Mouse (100%), Rat (100%), Japanese Medaka (98%), Xenopus laevis (99%), Dog (100%), Rabbit (99%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%), Guinea pig (100%).
Positive Control: 293T, A431, HeLa, HepG2, Neuro2A, CD830, NIH-3T3, Raw264.7, C2C12, mouse liver, rat liver, PC-12, Rat2, drosophila.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: actin, beta; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed; Atcb; beta cytoskeletal actin; Beta-actin; Cap Z; capZ beta; cytoplasmic beta-actin; cytoskeleton actin; gamma actin; PS1TP5-binding protein 1
基因别名: ACTB; Actx; beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1
UniProt ID: (Human) P60709, (Rat) P60711, (Mouse) P60710
Entrez Gene ID: (Human) 60, (Rat) 81822, (Mouse) 11461
分子生物学功能:
actin and actin related protein
actin or actin-binding cytoskeletal protein
cytoskeletal protein
