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GenScript
beta Actin Monoclonal Antibody (2D1D10), THE™
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beta Actin Antibody (A00702-100) in WB
Lane 1: 20 µg Hela cell lysate. Lane 2: 20 µg sp2/0 cell lysate. Lane 3: 20 µg goat muscle lysate. Lane 4: 20 µg rabbit muscle lysate. Lane 5: 20 µg chicken muscle lysate. Lane 6: 20 µg CHO cell lysate. Lane 7: 20 µg fish muscle lysate. Primary antibody: 1 µg/mL THE™ Anti-beta-actin Monoclonal Antibody (Mouse) (GenScript, A00702). Secondary antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:20,000).
产品信息
A00702-100
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Chicken,
Fish,
Goat,
Hamster,
Human,
Mouse,
Pig,
Rabbit
宿主/亚型
Mouse
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
2D1D10
抗原
A synthetic peptide DDDIAALVVDNGSG coupled to KLH
偶联物
形式
Lyophilized
纯化类型
Protein A
保存液
PBS, pH 7.4, with 1% BSA
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Reconstitute the lyophilized antibody with deionized water to the final concentration of 0.5 mg/mL.
beta-actin is one of the six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. As a consequence, it has been difficult to produce potent antisera against this protein. Therefore, the availability of monoclonal antibodies to beta-actin provides a useful and specific tool in the study of the intracellular distribution of β-actin and the static and dynamic aspects of the cytoskeleton.
Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below.
靶标信息
Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: actin, beta; Actin, cytoplasmic 1; Bact; actin; beta actin; beta cytoskeletal actin; Beta-actin; cytoplasmic 1; cytoskeletal beta actin; gamma non-muscle actin; PS1TP5-binding protein 1
基因别名: ACT-5; ACTB; actin; Actx; Bact; Beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1
protein binding
ATP binding
kinesin binding
Tat protein binding
identical protein binding
nitric-oxide synthase binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
structural constituent of cytoskeleton
nucleosomal DNA binding
protein kinase binding
structural constituent of postsynaptic actin cytoskeleton
actin and actin related protein
actin or actin-binding cytoskeletal protein
cytoskeletal protein
morphogenesis of a polarized epithelium
establishment or maintenance of cell polarity
regulation of transmembrane transporter activity
negative regulation of protein binding
adherens junction assembly
apical protein localization
synaptic vesicle endocytosis
regulation of norepinephrine uptake
protein localization to adherens junction
cellular response to cytochalasin B
postsynaptic actin cytoskeleton organization
regulation of protein localization to plasma membrane
retina homeostasis
movement of cell or subcellular component
cytoskeleton organization
substantia nigra development
cell junction assembly
Fc-gamma receptor signaling pathway involved in phagocytosis
ATP-dependent chromatin remodeling
positive regulation of gene expression, epigenetic
vascular endothelial growth factor receptor signaling pathway
ephrin receptor signaling pathway
platelet aggregation
axonogenesis
cell motility
