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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • beta Actin Antibodies

          Invitrogen

          beta Actin Loading Control Monoclonal Antibody (BA3R), DyLight™ 488

          高级验证
          7个已发表图片
          84 篇参考文献
          查看其他56个beta Actin抗体

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          说明书
          实验方案
          问题与答案
          技术支持
          说明书
          实验方案
          问题与答案
          技术支持

          Cite beta Actin Loading Control Monoclonal Antibody (BA3R), DyLight™ 488

          • 抗体检测数据 (2)
          • 已发表图片 (7)
          • 高级验证 (1)
          beta Actin Loading Control Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          beta Actin Loading Control Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          图: 1 / 10

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          beta Actin Loading Control Antibody (MA5-15739-D488) in ICC/IF

          Immunofluorescent analysis of beta-actin (green) in HeLa cells. Cells were fixed with either 4% paraformaldehyde (PFA) for 15 minutes at room temperature followed by permeabilization with 0.5% Triton X-100 in PBS for 15 minutes at room temperature (left panel) or fixed and permeabilized with ice-cold methanol for 10 minutes at room temperature (right panel). Cells were blocked with 0.3% BSA in PBS for 15 mi... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          查看产品

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          beta Actin Loading Control Antibody in Immunocytochemistry (ICC/IF)
          beta Actin Loading Control Antibody in Western Blot (WB)
          beta Actin Loading Control Antibody in Western Blot (WB)
          beta Actin Loading Control Antibody in Western Blot (WB)
          beta Actin Loading Control Antibody in Western Blot (WB)
          beta Actin Loading Control Antibody in Western Blot (WB)
          beta Actin Loading Control Antibody in Western Blot (WB)
          beta Actin Loading Control Antibody in Western Blot (WB)
          beta Actin Loading Control Antibody in Western Blot (WB)
          beta Actin Loading Control Antibody

          产品信息

          MA5-15739-D488

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:1,000
          查看84篇已发表文章 84篇已发表文章

          免疫细胞化学 (ICC/IF)

          1:20 - 1:100
          -
          产品规格

          种属反应

          Chicken, Human, Mouse, Rabbit, Rat

          已发表种属

          Guinea pig, Human, Mouse, Plant, Rabbit, Rat

          宿主/亚型

          Mouse / IgG2b

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          BA3R

          抗原

          Beta-actin N-terminal peptide.
          查看免疫原

          偶联物

          DyLight™ 488 DyLight™ 488 DyLight™ 488
          • Unconjugated
          • Biotin 
          • DyLight 550
          • DyLight 650
          • DyLight 680
          • DyLight 800 4X PEG
          • HRP
          • Request custom conjugation

          激发/发射光谱

          492/519 nm 查看光谱 spectra

          形式

          Liquid

          浓度

          1 mg/mL

          纯化类型

          Protein A

          保存液

          sodium bicarbonate

          内含物

          no preservative

          保存条件

          4°C

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2537662

          产品详细信息

          MA5-15739-D488 detects beta actin from human, mouse, rat, rabbit, and chicken samples.

          MA5-15739-D488 has been successfully used in immunofluorescence. DyLight 488 has an excitation/emission of 493/518 nm.

          For visualization of actin filaments by immunofluorescence, fixation and permeabilization with methanol is required.

          靶标信息

          Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: actin, beta; Actin, cytoplasmic 1; Bact; actin; beta cytoskeletal actin; Beta-actin; cytoplasmic 1; cytoplasmic beta-actin; gamma non-muscle actin; PS1TP5-binding protein 1

          查看更多 收起

          基因别名: ACT-5; ACTB; actin; Actx; Bact; beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1

          查看更多 收起

          UniProt ID: (Rabbit) P29751, (Human) P60709, (Chicken) P60706, (Rat) P60711, (Mouse) P60710

          查看更多 收起

          Entrez Gene ID: (Rabbit) 100009272, (Human) 60, (Chicken) 396526, (Rat) 81822, (Mouse) 11461

          查看更多 收起

          功能
          RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding structural constituent of cytoskeleton protein binding ATP binding kinesin binding Tat protein binding nucleosomal DNA binding identical protein binding nitric-oxide synthase binding protein kinase binding structural constituent of postsynaptic actin cytoskeleton actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein
          参与通路
          retina homeostasis movement of cell or subcellular component cytoskeleton organization substantia nigra development cell junction assembly Fc-gamma receptor signaling pathway involved in phagocytosis ATP-dependent chromatin remodeling positive regulation of gene expression, epigenetic vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway platelet aggregation axonogenesis cell motility postsynaptic actin cytoskeleton organization retina development in camera-type eye cellular response to electrical stimulus
          It has to be done as per old AB suggested Products section.
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          性能保障

          只要您发现Invitrogen抗体在实验中的表现与网站或说明书的所述内容不符,您即可享受免费退换货服务。*

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