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Invitrogen
beta-Catenin Polyclonal Antibody
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beta-Catenin Antibody (AHO0462) in ICC/IF
Immunofluorescent analysis of Catenin beta (green) in HeLa cells either left untreated (left panel) or treated with 10uM GSK-3 Inhibitor X for 20 hours (right panel). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a Catenin beta polyclonal ant... View More
产品信息
AHO0462
应用
建议稀释比
已发表文章
产品规格
已发表种属
Chicken,
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide from the C-terminus of the beta-catenin protein
偶联物
形式
Liquid
浓度
0.2 mg/mL
保存条件
4°C
运输条件
Wet ice (domestic); Wet or Dry ice (international)
RRID
产品详细信息
This antibody reacts with the C-terminal portion of beta-Catenin.
For staining of formalin-fixed, paraffin-embedded tissues, it is recommended to boil tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at room temperature for 20 min. Recommended positive controls are MCF-7 cells and breast carcinoma tissue.
靶标信息
Beta-catenin, an adherens junction (AJ) protein, was originally identified as a component of cell-cell adhesion structures. AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. Beta-catenin interacts with the cytoplasmic domain of E-cadherin and links E-cadherin to alpha-catenin, which in turn mediates anchorage of the E-cadherin complex to the cortical actin cytoskeleton. Studies show that Beta-catenin also binds to another cytoskeletal complex containing the adenomatous polyposis coli protein and microtubules, and interacts with several signaling pathways that include tyrosine kinases, phosphatases and Wnt/Wingless. The interplay between beta-catenin, cytoskeletal complexes and signaling pathways may regulate morphogenesis. Beta-catenin is expressed in several hair follicle cell types, basal and peripheral matrix cells, and cells of the outer and inner root sheats. A pathological role of beta-catenin has been identified in pilomatrixoma (PTR), medulloblastoma (MDB), colorectal cancer (CRC), ovarian cancer, and tumor development. In the nucleus, beta-catenin serves to co activate a family of Lef/Tcf transcription factors that stimulate transcription of target genes including those encoding cyclin D and c-myc that promote cell proliferation. The influence on cell proliferation is the molecular basis for the role of beta-catenin in tumorgenesis, specifically, solid tumors of the breast, colon, liver, lung, gastric, prostate, and skin.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
