Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫印迹 (WB) |
1:400 | 查看1篇已发表文章 1篇已发表文章 |
免疫组化 (IHC) |
Assay-Dependent | 查看3篇已发表文章 3篇已发表文章 |
免疫组化（冰冻） (IHC (F)) |
- | 查看1篇已发表文章 1篇已发表文章 |
免疫组织化学（悬浮细胞） (IHC (Free)) |
- | 查看1篇已发表文章 1篇已发表文章 |
| 产品规格 | |
|---|---|
种属反应 |
Bacteria |
已发表种属 |
Bacteria, Fruit fly, Mouse |
宿主/亚型 |
Rabbit / IgG |
分类 |
Polyclonal |
类型 |
Antibody |
偶联物 |
Unconjugated |
形式 |
Liquid |
浓度 |
0.2 mg/mL |
纯化类型 |
Affinity chromatography |
保存液 |
PBS |
内含物 |
0.1% sodium azide |
保存条件 |
4° C |
RRID |
AB_468331 |
Description: The rabbit polyclonal antibody reacts with bBeta galactosidase. Beta-galactosidase is a large 464 kDa tetramer when assayed by western blot under nonreducing conditions and 116 kDa monomer under reducing conditions. The beta-galactosidase activity allows for the quick determination of cells expressing the lacZ gene. The lacZ gene is used as a reporter construct for determination of transfection efficiency as well as histochemical localization following transfection of eukaryotic cells. The lacZ gene product beta-galactosidase catalyzes the hydrolysis of the substrate 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal) to produce a blue color that is easily visualized with a microscope.
Applications Reported: Purified Anti-beta galactosidase fusion proteins poly has been reported for use in immunoblotting (WB), and immunohistochemical staining.
Applications Tested: Purified Anti-beta galactosidase fusion proteins poly has been tested by immunoblotting (WB). (1:400 starting dilution). It is recommended that this antibody be titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Beta Galactosidase is an exoglycosidase which hydrolyzes the beta-glycosidic bond formed between a galactose and its organic moiety. Deficiencies in the protein in humans can result in galactosialidosis or Morquio B syndrome. In E. coli, the gene of beta-galactosidase, the lacZ gene, is present as part of the inducible system lac operon which is activated in the presence of lactose when glucose level is low. E. coli beta Galactosidase is commonly used in molecular biology as a reporter marker to monitor gene expression. Another popular use for beta Galactosidase is in blue/white screening to identify recombinant clones. Beta Galactosidase can be split in two peptides, lacZalpha and LacZOmega, neither of which is active by itself but when both are present together, spontaneously reassemble into a functional enzyme. This property is exploited in many cloning vectors. The presence or absence of an active beta Galactosidase may be detected through addition of artificial chromogenic substrates such as X-gal, fluorescent substrates such as Fluorescein di-beta-D-galactopyranoside (FDG), luminescent substrates and others. Beta Galactosidase activity at pH 6 is an indicator of senescent cells not found in presenescent, quiescent or dividing cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: Beta-Gal; betagal; bgal; ß-gal; ß-galactosidase; ßgal
