Invitrogen

c-Kit Monoclonal Antibody (104D2), Alexa Fluor™ 700

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c-Kit Antibody (MA562186) in Flow

Flow cytometry (multicolor surface staining) of c-Kit in human leukocytes. Samples were stained with c-Kit monoclonal antibody (Product # MA562186) using a dilution of 4 µL reagent/100 µL of peripheral whole blood with anti-human CD34 (581) PE antibody at 20 µL reagent/100 µL of peripheral whole blood.

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产品信息

MA562186

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

4 µL/100 µL whole blood or 10^6 cells

产品规格

种属反应

Bovine, Human, Non-human primate

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

104D2

抗原

MOLM-1 megakaryocytic cells.

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偶联物

Alexa Fluor™ 700 Alexa Fluor™ 700 Alexa Fluor™ 700

激发/发射光谱

696/719 nm 查看光谱

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Size-exclusion chromatography

保存液

PBS, pH 7.4, with 0.2% BSA

内含物

15mM sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: c-kit receptor; CD117; ckit; mast cell growth factor receptor; Mast/stem cell growth factor receptor Kit; p145 c-kit; PBT; Piebald trait protein; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

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基因别名: C-Kit; CD117; KIT; PBT; SCFR

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UniProt ID: (Human) P10721

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Entrez Gene ID: (Bovine) 280832, (Human) 3815

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