Invitrogen

c-Rel Recombinant Rat Monoclonal Antibody (1RELAH5)

图: 1 / 3

c-Rel Antibody (740087T) in WB

Western blot was performed using c-Rel Recombinant Rat Monoclonal Antibody (1RELAH5) (Product # 740087T, 1:500) and a ~65 kDa band corresponding to c-Rel was observed across cell lines tested. Whole cell extracts (30 µg lysate) of EL4 (Lane 1), RAW 264.7 (Lane 2) and NIH/3T3 (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel, (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot™ 2 Dry Blotting System (Product # IB21001) and probed with the primary ant... View More

Product Image Gallery

c-Rel Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Expression of c-Rel was observed specifically in EL4 and RAW 264.7 cells, but not in NIH/3T3 cells when probed with c-Rel Recombinant Rat Monoclonal Antibody (1RELAH5) (Product # 740087T) in Western blot. {RE}

产品信息

740087T

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

流式细胞分析 (Flow)

0.5 µg/test

产品规格

种属反应

Mouse

宿主/亚型

Rat / IgG2a, kappa

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

1RELAH5

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.0 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

产品详细信息

Recombinant rat monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity, lot-to-lot consistency, and broader immunoreactivity to diverse targets due to a larger rabbit immune repertoire.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

靶标信息

The REL gene encodes c-Rel, a transcription factor that is a member of the Rel/NFKB family, which also includes RELA., RELB (604758), NFKB1, and NFKB2. These proteins are related through a highly conserved N-terminal region termed the 'Rel domain, ' which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFKB inhibitor (Belguise and Sonenshein, 2007).

仅用于科研。不用于诊断过程。未经明确授权不得转售。