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Agrisera
eEF1B-gamma1 and 2/elongation factor 1-gamma 1 and 2 Polyclonal Antibody
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eEF1B-gamma1 and 2/elongation factor 1-gamma 1 and 2 Antibody (AS10-676) in WB
10 µg total protein extracted from 15 day old Arabidopsis thaliana seedlings that were either kept at 22ºC (1) or heat treated at 38ºC for 2 hours prior to protein extraction (2). As positive control 10 ng of recombinant elongation factor proteins gamma 1 (3) and gamma 2 (4) were separated side by side with the plant samples on 11% SDS-PAGE and blotted to nitrocellulose (Bio-Rad). Blots were blocked followi... View More
产品信息
AS10-676
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Recombinant eEF1B-gamma1 protein from Arabidopsis thaliana with no affinity tag.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 200 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
EF1G1 encodes a ubiquitously expressed, predominantly cytoplasmic protein that also partitions to the nucleus and associates with ribosome-rich compartments, functioning as the gamma subunit of the eukaryotic elongation factor 1 (eEF1) complex. The protein is a ~437 amino acid polypeptide with an N-terminal glutathione S-transferase-like module (GST-like fold) followed by a conserved C-terminal elongation factor 1 gamma domain, an architecture consistent with a scaffold/adaptor role within multi-protein assemblies. Biologically, EF1G1 contributes to translation elongation by participating in the eEF1 machinery that supports efficient delivery of aminoacyl-tRNAs to the ribosomal A site through regulation/organization of the eEF1 subunits; its GST-like region is proposed to help organize or stabilize multisubunit complexes, including those linking elongation factors with aminoacyl-tRNA synthetase assemblies, thereby coordinating high-throughput protein synthesis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: At1g09640
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