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Invitrogen
eIF1 Polyclonal Antibody
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eIF1 Antibody (PA5-143916) in ICC/IF
Immunocytochemistry analysis of eIF1 in T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in eIF1 Polyclonal antibody (Product # PA5-143916) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate fo... View More
产品信息
PA5-143916
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human EIF1 recombinant protein (Position: S2-D106).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human HEK293 whole cell, human Jurkat whole cell, human Raji whole cell, human K562 whole cell, rat pancreas tissue, mouse pancreas tissue, mouse NIH/3T3 whole cell. IHC: human placenta tissue. ICC/IF: T-47D cell. Flow: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
Necessary for scanning and involved in initiation site selection. Promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: A121; eIF1; eIF13; Eukaryotic translation initiation factor 1; Protein translation factor SUI1 homolog; Sui1iso1; suppressor of initiator codon mutations, related sequence 1; suppressor of initiator codon mutations-Yeast homolog related sequence 1
基因别名: A121; EIF-1; EIF1; EIF1A; ISO1; SUI1; Sui1-rs1
UniProt ID: (Human) P41567, (Mouse) P48024
Entrez Gene ID: (Human) 10209, (Rat) 287703, (Mouse) 20918
