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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • eIF3e Antibodies

          Invitrogen

          eIF3e Polyclonal Antibody

          View all (14) eIF3e antibodies
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          Cite eIF3e Polyclonal Antibody

          Datasheet
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          • Antibody Testing Data (1)
          eIF3e Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          eIF3e Antibody (PA5-95431) in WB

          Western blot analysis of EIF3e in, Lane 1: rat thymus tissue lysates, Lane 2: mouse thymus tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: COLO320 whole cell lysates, Lane 5: HELA whole Cell lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. The membrane was blocked ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Product Details

          PA5-95431

          Applications
          Tested Dilution
          Publications

          免疫印迹 (WB)

          0.1-0.5 µg/mL
          -
          产品规格

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          分类

          Polyclonal

          类型

          Antibody

          Immunogen

          E.coli-derived human EIF3e recombinant protein (Position: A160-Q241).
          View immunogen

          Conjugate

          Unconjugated

          形式

          Lyophilized

          浓度

          500 µg/mL

          纯化类型

          Affinity chromatography

          保存液

          PBS with 4mg trehalose

          内含物

          0.05mg sodium azide

          保存条件

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          RRID

          AB_2807234

          Product Specific Information

          Human EIF3e shares 100% amino acid (aa) sequence identity with both mouse and rat EIF3e.

          Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

          Target Information

          Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: eIF-3 p48; EIF3E; Eukaryotic translation initiation factor 3 subunit 6; Eukaryotic translation initiation factor 3 subunit E; eukaryotic translation initiation factor 3 subunit E isoform 2 transcript; eukaryotic translation initiation factor 3, subunit 6 (48kD); eukaryotic translation initiation factor 3, subunit 6 48kDa; eukaryotic translation initiation factor 3, subunit E; mammary tumor integration site 6; Mammary tumor-associated protein INT-6; mammary tumor-associated protein INT6; MMTV integration site 6; murine mammary tumor integration site 6 (oncogene homolog); Viral integration site protein INT-6; Viral integration site protein INT-6 homolog

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          Gene Aliases: 48kDa; eIF3-p46; EIF3-P48; EIF3E; EIF3S6; INT6

          View more View less

          UniProt ID: (Human) P60228, (Mouse) P60229

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          Entrez Gene ID: (Human) 3646, (Mouse) 16341, (Rat) 103692906

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          Molecular Function: translation initiation factor

          Function(s)
          nuclear-transcribed mRNA catabolic process, nonsense-mediated decay formation of translation preinitiation complex translational initiation regulation of translational initiation negative regulation of translational initiation cell-cell adhesion translation
          Process(es)
          translation initiation factor activity protein binding poly(A) RNA binding protein N-terminus binding cadherin binding involved in cell-cell adhesion
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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