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Invitrogen
eIF4E Recombinant Rabbit Monoclonal Antibody (eIF4E-Clone 155)
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eIF4E Antibody (704989) in ICC/IF
Immunofluorescence analysis of eIF4E was performed using eIF4E Recombinant Rabbit Monoclonal Antibody (eIF4E-Clone 155) (Product # 704989) on 70-80% confluent U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 mins, permeabilized with 0.1% Triton X-100 for 15 mins and blocked with 2% BSA for 1 hr at room temperature. The cells were stained with eIF4E Recombinant Rabbit Monoclonal Antibody (eIF4E-Clone 155) (Product # 704989, 1:200) in 0.1% BSA, incubated at 4 degree Celsius overnight, followed by labelling with Donkey anti... View More
产品信息
704989
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
eIF4E-Clone 155
抗原
Protein corresponding to human eIF4E [aa1-aa217]
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
产品详细信息
Recombinant Rabbit monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
靶标信息
eIF4E is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A, that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: eIF-4E; eIF-4F 25 kDa subunit; Eukaryotic translation initiation factor 4E; eukaryotic translation initiation factor 4E-like 1; MGC111573; mRNA cap-binding protein; OTTHUMP00000219703; OTTHUMP00000219704; OTTHUMP00000219705; unnamed protein product
基因别名: AUTS19; CBP; eIF-4E; EIF4E; EIF4E1; EIF4EL1; EIF4F
UniProt ID: (Human) P06730
Entrez Gene ID: (Human) 1977
G1/S transition of mitotic cell cycle
behavioral fear response
mRNA export from nucleus
translation
translational initiation
regulation of translation
negative regulation of autophagy
negative regulation of translation
stem cell population maintenance
neuron differentiation
negative regulation of neuron differentiation
positive regulation of mitotic cell cycle
mRNA transport
nuclear export
cellular response to dexamethasone stimulus
regulation of translation at postsynapse, modulating synaptic transmission
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