Invitrogen

eRF1 Monoclonal Antibody (3E5)

Name: eRF1 Monoclonal Antibody (3E5)
Brand: Invitrogen
SKU: MA5-49272
Availability: InStock

查看其他10个eRF1抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (14)

eRF1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 14

eRF1 Antibody (MA5-49272) in ICC/IF

Immunocytochemistry analysis of eRF1 in A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in eRF1 Monoclonal antibody (Product # MA5-49272) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for t... View More

eRF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Immunogen image

Immunogen Image eRF1 Monoclonal Antibody (3E5)

产品信息

MA5-49272

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.25-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

2-5 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

1-3 µg/10x^6 cells

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

3E5

抗原

E.coli-derived human eRF1/ETF1 recombinant protein (Position: D9-K342).

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

100 µg

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_3074930

产品详细信息

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Positive Control - WB: human Hela whole cell, human Jurkat whole cell, human K562 whole cell, human Raji whole cell, human HEPG2 whole cell, human HEPG2 whole cell, rat liver tissue, rat testis tissue, rat kidney tissue, rat PC-12 whole cell, mouse liver tissue, mouse testis tissue, mouse kidney tissue, mouse RAW2647 whole cell. IHC: human tonsil tissue, human breast cancer tissue, human liver cancer tissue, human lung cancer tissue, rat pancreas tissue. ICC/IF: A431 cell. Flow: Caco-2 cell, HEPA1-6 cell, RH35 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors.

仅用于科研。不用于诊断过程。未经明确授权不得转售。