应用 | 建议稀释比 | 已发表文章 |
---|---|---|
免疫印迹 (WB) |
1 µg/mL | - |
免疫组化(石蜡) (IHC (P)) |
1 µg/mL | - |
免疫细胞化学 (ICC/IF) |
Assay-dependent | - |
酶联免疫吸附实验 (ELISA) |
Assay-dependent | - |
免疫沉淀 (IP) |
Assay-dependent | - |
产品规格 | |
---|---|
种属反应 |
Human, Mouse, Xenopus |
宿主/亚型 |
Mouse / IgG1 |
分类 |
Monoclonal |
类型 |
Antibody |
克隆号 |
1G11 |
抗原 |
proprietary immunogen |
偶联物 |
Unconjugated |
形式 |
Liquid |
浓度 |
1 mg/mL |
纯化类型 |
Protein A |
保存液 |
PBS |
内含物 |
0.1% sodium azide |
保存条件 |
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C |
RRID |
AB_2539408 |
For IHC applications, it is necessary to perform heat-mediated antigen retrieval with citrate buffer, pH 6, prior to staining. In Western blot applications, this antibody detects a band at ~53 kDa (hnRNP F, predicted 49.8 kDa) and 56 kDa (hnRNP H, predicted 53.9 kDa).
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins. The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA. These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: Heterogeneous nuclear ribonucleoprotein F; Heterogeneous nuclear ribonucleoprotein F, N-terminally processed; hnRNP F; HnRNP F protein; HNRNPH1; mcs94 1; Nucleolin-like protein mcs94-1; ROF
基因别名: 4833420I20Rik; AA407306; HNRNPF; HNRPF; mcs94-1; OK/SW-cl.23
UniProt ID: (Human) P52597, (Mouse) Q9Z2X1
Entrez Gene ID: (Human) 3185, (Mouse) 98758
分子生物学功能:
RNA splicing factor