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产品信息
31181
应用
建议稀释比
免疫印迹 (WB)
Assay-dependent
免疫组化 (IHC)
Assay-dependent
免疫细胞化学 (ICC/IF)
Assay-dependent
酶联免疫吸附实验 (ELISA)
Assay-dependent
In Situ Hybridization (ISH)
Assay-dependent
产品规格
种属反应
Mouse
宿主/亚型
Horse
/ IgG
分类
Polyclonal
类型
Secondary Antibody
偶联物
形式
Liquid
浓度
3 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.8, with 20mg/mL sucrose
内含物
0.08% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_228364
靶标
IgG
抗体形式
Whole Antibody
产品详细信息
Product # 31181 has been successfully used in Western blot, IF, ICC, IHC, ELISA and ISH applications.
Product # 31181 reacts with heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins. Based on solid-phase enzyme immunoassay, this antibody cross-reacts with rat IgG and mouse IgM (25%), hamster IgG (12%), guinea pig IgG and rat IgM (6%), bovine IgM, dog IgM, goat IgM, human IgM, Rhesus monkey IgG, sheep IgG, sheep IgM, swine IgG (1%). It has minimum cross-reactivity with immunoglobulins from other species.
The activity measured by a solid phase binding assay is 94%.
靶标信息
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
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