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          • Primary Antibodies ›
          • mTOR Antibodies
          Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

          Invitrogen

          mTOR Recombinant Superclonal™ Antibody (7HCLC)

          高级验证
          查看其他56个mTOR抗体

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          Cite mTOR Recombinant Superclonal™ Antibody (7HCLC)

          Additional Information:
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          • 抗体检测数据 (5)
          • 高级验证 (1)
          mTOR Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          mTOR Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          图: 1 / 6

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          mTOR Antibody (710408) in ICC/IF

          Immunofluorescence analysis of mTOR was performed using 70% confluent log phase HCT 116 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with mTOR Recombinant Rabbit Superclonal™ Antibody (7HCLC) (Product # 710408, 0.5 µg/mL) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plu... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
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          查看产品

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          mTOR Antibody in Immunocytochemistry (ICC/IF)
          mTOR Antibody in Immunocytochemistry (ICC/IF)
          mTOR Antibody in Western Blot (WB)
          mTOR Antibody in Western Blot (WB)
          mTOR Antibody in ChIP Assay (ChIP)
          mTOR Antibody

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          mTOR Recombinant Superclonal™ Antibody (7HCLC)

          产品信息

          710408

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          2-3 µg/mL
          -

          免疫细胞化学 (ICC/IF)

          0.5 µg/mL
          -

          染色质免疫沉淀 实验 (ChIP)

          1 µL
          -
          产品规格

          种属反应

          Human, Rat

          宿主/亚型

          Rabbit / IgG

          Expression System

          Expi293

          分类

          Recombinant Superclonal

          类型

          Antibody

          克隆号

          7HCLC

          抗原

          Recombinant protein corresponding to amino acids 1126-1411 of human mTOR.
          3D表位/免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          0.5 mg/mL

          规格

          100 µg

          纯化类型

          Protein A

          保存液

          PBS, pH 7.2-7.4

          内含物

          0.09% sodium azide

          保存条件

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          运输条件

          Wet ice

          RRID

          AB_2532712

          产品详细信息

          Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

          靶标信息

          FRAP1 (mTOR) is a serine/threonine kinase that plays a critical role in cellular growth and proliferation. Perturbations in the mTOR/PI3-kinase/AKT pathway are associated with numerous forms of cancer. FRAP1 is also the target of rapamycin and its analogues, which are currently used as immunosuppressants and cancer therapeutics. Mutations affecting the gene results in Smith-Kingsmore syndrome.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          篇参考文献 (0)

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          引用该产品

          生物信息学

          蛋白别名: FK506 binding protein 12-rapamycin associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP-rapamycin associated protein; FKBP12-rapamycin complex-associated protein; FKBP12-rapamycin complex-associated protein 1; m-TOR; mammalian target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); mTORC1; rapamycin and FKBP12 target 1; rapamycin and FKBP12 target-1 protein; rapamycin associated protein FRAP2; rapamycin target protein 1; RAPT1; tyrosine-protein kinase mTOR; unnamed protein product

          查看更多 收起

          基因别名: FRAP; FRAP1; FRAP2; RAFT1; RAPT1; SKS

          查看更多 收起

          UniProt ID: (Human) P42345, (Rat) P42346

          查看更多 收起

          Entrez Gene ID: (Human) 2475, (Rat) 56718

          查看更多 收起

          功能
          inositol hexakisphosphate binding RNA polymerase III type 1 promoter sequence-specific DNA binding RNA polymerase III type 2 promoter sequence-specific DNA binding RNA polymerase III type 3 promoter sequence-specific DNA binding TFIIIC-class transcription factor binding protein kinase activity protein serine/threonine kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity protein binding kinase activity identical protein binding ribosome binding ion channel binding macromolecular complex binding phosphoprotein binding protein serine kinase activity protein kinase binding protein domain specific binding
          参与通路
          regulation of cell growth T-helper 1 cell lineage commitment 'de novo' pyrimidine nucleobase biosynthetic process protein phosphorylation inflammatory response cellular response to DNA damage stimulus cytoskeleton organization regulation of cell size cellular response to starvation response to heat regulation of autophagy negative regulation of autophagy positive regulation of epithelial to mesenchymal transition regulation of macroautophagy negative regulation of macroautophagy regulation of lipid metabolic process neuronal action potential positive regulation of cell growth T cell costimulation response to nutrient levels cellular response to nutrient levels cellular response to nutrient TOR signaling cellular response to insulin stimulus regulation of actin cytoskeleton organization cellular response to amino acid starvation TORC1 signaling TORC2 signaling regulation of circadian rhythm negative regulation of apoptotic process response to amino acid anoikis protein kinase B signaling positive regulation of cell differentiation regulation of osteoclast differentiation positive regulation of translation positive regulation of glycolytic process positive regulation of protein kinase activity positive regulation of transcription from RNA polymerase III promoter positive regulation of translational initiation negative regulation of insulin receptor signaling pathway positive regulation of lipid biosynthetic process behavioral response to pain protein stabilization positive regulation of multicellular organismal process positive regulation of keratinocyte migration regulation of protein kinase B signaling positive regulation of protein kinase B signaling cardiac cell development cellular response to methionine positive regulation of SCF-dependent proteasomal ubiquitin-dependent catabolic process cellular response to amino acid stimulus cellular response to leucine cellular response to hypoxia cellular response to osmotic stress regulation of cellular response to stress vascular endothelial cell response to laminar fluid shear stress regulation of cellular response to heat negative regulation of protein localization to nucleus regulation of signal transduction by p53 class mediator positive regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter positive regulation of wound healing, spreading of epidermal cells regulation of locomotor rhythm positive regulation of cytoplasmic translational initiation regulation of lysosome organization negative regulation of lysosome organization positive regulation of pentose-phosphate shunt cellular response to leucine starvation positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process regulation of autophagosome assembly regulation of protein phosphorylation negative regulation of protein phosphorylation positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation heart morphogenesis heart valve morphogenesis regulation of glycogen biosynthetic process regulation of carbohydrate metabolic process energy reserve metabolic process germ cell development long-term memory visual learning response to virus post-embryonic development positive regulation of lamellipodium assembly positive regulation of myotube differentiation positive regulation of neuron projection development positive regulation of neuron maturation negative regulation of muscle atrophy response to activity peptidyl-serine phosphorylation peptidyl-threonine phosphorylation spinal cord development cell projection organization positive regulation of actin filament polymerization positive regulation of cell projection organization negative regulation of protein ubiquitination regulation of myelination regulation of fatty acid beta-oxidation regulation of response to food response to insulin social behavior multicellular organism growth response to cocaine positive regulation of neuron apoptotic process regulation of carbohydrate utilization positive regulation of nitric oxide biosynthetic process negative regulation of cell size regulation of protein kinase activity protein autophosphorylation mRNA stabilization positive regulation of smooth muscle cell proliferation positive regulation of oligodendrocyte differentiation cardiac muscle tissue development positive regulation of peptidyl-tyrosine phosphorylation positive regulation of neurogenesis regulation of behavior voluntary musculoskeletal movement positive regulation of striated muscle cell differentiation positive regulation of stress fiber assembly cardiac muscle cell development cardiac muscle contraction maternal process involved in female pregnancy positive regulation of glial cell proliferation positive regulation of dendritic spine development positive regulation of cell growth involved in cardiac muscle cell development negative regulation of calcineurin-NFAT signaling cascade regulation of brown fat cell differentiation regulation of membrane permeability regulation of translation at synapse, modulating synaptic transmission regulation of plasma membrane bounded cell projection organization positive regulation of ruffle assembly positive regulation of eating behavior positive regulation of epithelial cell apoptotic process positive regulation of cholangiocyte proliferation negative regulation of cholangiocyte apoptotic process positive regulation of granulosa cell proliferation positive regulation of skeletal muscle hypertrophy negative regulation of iodide transmembrane transport positive regulation of cellular senescence
          It has to be done as per old AB suggested Products section.

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