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Invitrogen
mazF Polyclonal Antibody
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mazF Antibody (PA5-144386) in WB
Western blot analysis of mazF in recombinant protein using a dilution of 1:2,000. Samples were incubated with mazF polyclonal antibody (Product # PA5-144386). Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 29 kDa. Observed band size: 29 kDa.
产品信息
PA5-144386
产品规格
种属反应
Bacteria
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant Escherichia coli O157:H7 mazF protein (1-111aa )
偶联物
形式
Liquid
浓度
4 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.03% Proclin 300
保存条件
-20°C or -80°C if preferred
运输条件
Ambient (domestic); Wet ice (international)
RRID
靶标信息
Toxic component of a type II toxin-antitoxin (TA) system. MazF is a sequence-specific endoribonuclease that inhibits protein synthesis and induces bacterial stasis. It is very stable, single-strand specific and cleavage is independent of the ribosome. The endoribonuclease activity (a toxin) is inhibited by the labile cognate antitoxin MazE. Toxicity results when the levels of MazE decrease in the cell, leading to mRNA degradation. Both MazE and MazE-MazF bind to the promoter region of the mazE-mazF operon to inhibit their transcription.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: chpA
