Invitrogen

p53 Monoclonal Antibody (DO-7)

高级验证
24个已发表图片
176 篇参考文献
查看其他166个p53抗体

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

说明书
实验方案
问题与答案
技术支持
说明书
实验方案
问题与答案
技术支持

产品信息

MA5-12557

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:500-1:4,000
查看51篇已发表文章 51篇已发表文章

免疫组化 (IHC)

-
查看102篇已发表文章 102篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:200
查看5篇已发表文章 5篇已发表文章

免疫组化（冰冻） (IHC (F))

-
查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:20-1:200
查看8篇已发表文章 8篇已发表文章

流式细胞分析 (Flow)

1:50
查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

1:50-1:100
查看3篇已发表文章 3篇已发表文章

染色质免疫沉淀 实验 (ChIP)

1 µg/mL
-

其他PubMed (Misc)

-
查看5篇已发表文章 5篇已发表文章
产品规格

种属反应

Bovine, Human

已发表种属

Human, Mouse, Rat

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

DO-7

抗原

Recombinant human wild-type p53 protein expressed in E. coli

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.05 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10989883

产品详细信息

MA5-12557 targets p53 in FACS, ICC/IF, IHC (P), IP, ChIP, and WB applications and shows reactivity with Human and bovine samples. This antibody is not suitable for mouse colon tissue in IHC applications.

The MA5-12557 immunogen is recombinant human wild-type p53 protein expressed in E. coli.

This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.

靶标信息

The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Antigen NY-CO-13; Cellular tumor antigen p53; Cys 51 Stop; FLJ92943; HGNC11998; mutant tumor protein 53; OTTMUSP00000006194; p53; p53 tumor suppressor; p53 tumor suppressor phosphoprotein; Phosphoprotein p53; transformation-related protein 53; tumor protein 53; tumor protein p53 (Li-Fraumeni syndrome); Tumor suppressor p53; tumor supressor p53; Tumour Protein p53

查看更多 收起

基因别名: BCC7; LFS1; P53; TP53; TRP53

查看更多 收起

UniProt ID: (Bovine) P67939, (Human) P04637

查看更多 收起

Entrez Gene ID: (Bovine) 281542, (Human) 7157

查看更多 收起

功能
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter sequence-specific DNA binding RNA polymerase II transcription factor binding TFIID-class transcription factor binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding protease binding p53 binding DNA binding mRNA 3'-UTR binding copper ion binding ATP binding receptor tyrosine kinase binding ubiquitin protein ligase binding histone deacetylase regulator activity histone acetyltransferase binding identical protein binding histone deacetylase binding protein self-association protein heterodimerization activity protein N-terminus binding chaperone binding protein phosphatase 2A binding MDM2/MDM4 family protein binding disordered domain specific binding promoter-specific chromatin binding chromatin binding damaged DNA binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding zinc ion binding enzyme binding protein kinase binding protein phosphatase binding sequence-specific DNA binding transcription regulatory region DNA binding P53-like transcription factor
参与通路
negative regulation of transcription from RNA polymerase II promoter DNA strand renaturation in utero embryonic development somitogenesis release of cytochrome c from mitochondria T cell proliferation involved in immune response B cell lineage commitment T cell lineage commitment response to ischemia nucleotide-excision repair double-strand break repair protein import into nucleus autophagy cellular response to DNA damage stimulus DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator ER overload response transforming growth factor beta receptor signaling pathway Ras protein signal transduction gastrulation negative regulation of neuroblast proliferation cell aging negative regulation of DNA replication determination of adult lifespan mRNA transcription rRNA transcription response to salt stress response to X-ray positive regulation of cardiac muscle cell apoptotic process viral process cerebellum development negative regulation of cell growth negative regulation of transforming growth factor beta receptor signaling pathway positive regulation of histone deacetylation chromatin assembly T cell differentiation in thymus regulation of tissue remodeling multicellular organism growth cellular response to drug positive regulation of mitochondrial membrane permeability cellular response to glucose starvation intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator positive regulation of apoptotic process negative regulation of apoptotic process entrainment of circadian clock by photoperiod mitochondrial DNA repair regulation of DNA damage response, signal transduction by p53 class mediator positive regulation of neuron apoptotic process negative regulation of proteolysis negative regulation of transcription, DNA-templated positive regulation of RNA polymerase II transcriptional preinitiation complex assembly positive regulation of transcription from RNA polymerase II promoter negative regulation of fibroblast proliferation circadian behavior bone marrow development embryonic organ development positive regulation of peptidyl-tyrosine phosphorylation protein stabilization RNA polymerase II transcriptional preinitiation complex assembly protein tetramerization neuron apoptotic process negative regulation of telomerase activity hematopoietic stem cell differentiation interferon-gamma-mediated signaling pathway cardiac septum morphogenesis positive regulation of transcription from RNA polymerase II promoter in response to hypoxia intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress positive regulation of thymocyte apoptotic process necroptotic process cellular response to gamma radiation cellular response to UV-C mitotic cell cycle arrest cellular response to actinomycin D positive regulation of release of cytochrome c from mitochondria positive regulation of cell aging replicative senescence oxidative stress-induced premature senescence oligodendrocyte apoptotic process positive regulation of execution phase of apoptosis negative regulation of macromitophagy regulation of mitochondrial membrane permeability involved in apoptotic process regulation of intrinsic apoptotic signaling pathway by p53 class mediator positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of production of miRNAs involved in gene silencing by miRNA positive regulation of production of miRNAs involved in gene silencing by miRNA negative regulation of glucose catabolic process to lactate via pyruvate negative regulation of pentose-phosphate shunt intrinsic apoptotic signaling pathway in response to hypoxia regulation of transcription from RNA polymerase II promoter in response to DNA damage positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress regulation of fibroblast apoptotic process negative regulation of reactive oxygen species metabolic process positive regulation of reactive oxygen species metabolic process regulation of cellular senescence positive regulation of intrinsic apoptotic signaling pathway base-excision repair regulation of transcription, DNA-templated transcription from RNA polymerase II promoter protein complex assembly cell cycle arrest multicellular organism development protein localization cell proliferation negative regulation of cell proliferation response to gamma radiation positive regulation of gene expression protein sumoylation cell differentiation DNA damage response, signal transduction by p53 class mediator mitotic G1 DNA damage checkpoint positive regulation of protein oligomerization cellular response to UV regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of transcription, DNA-templated response to antibiotic positive regulation of protein export from nucleus regulation of mitochondrial membrane permeability negative regulation of helicase activity positive regulation of cell cycle arrest cellular response to hypoxia cellular response to ionizing radiation intrinsic apoptotic signaling pathway by p53 class mediator intrinsic apoptotic signaling pathway positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway regulation of signal transduction by p53 class mediator regulation of cell cycle G2/M phase transition
guarantee_icon

性能保障

只要您发现Invitrogen抗体在实验中的表现与网站或说明书的所述内容不符，您即可享受免费退换货服务。*

了解更多
help_icon

随时为您服务

获取常见问题的专家解答，或者直接联系我们的技术专家，咨询应用、设备、常规产品使用问题。

联系技术支持