Invitrogen

pro-EGF Polyclonal Antibody

查看其他2个pro-EGF抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (2)

pro-EGF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

pro-EGF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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pro-EGF Antibody (PA5-29229) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human breast cancer, using EGF (Product # PA5-29229) antibody at 1:250 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

pro-EGF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

pro-EGF Antibody in Western Blot (WB)

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pro-EGF Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human breast cancer, using EGF (Product # PA5-29229) antibody at 1:250 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

pro-EGF Antibody in Western Blot (WB) — Western blot analysis of pro-EGF was performed by separating 50 µg of rat tissue extract by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a pro-EGF Polyclonal Antibody (Product # PA5-29229) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

产品信息

PA5-29229

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

-

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

-

产品规格

种属反应

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the Extracellular domain of human pro-EGF. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 10% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Predicted reactivity: Rat (80%), Dog (85%), Cat (86%), Pig (85%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: beta-urogastrone; EGF; Pro-epidermal growth factor

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基因别名: EGF; HOMG4; URG

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UniProt ID: (Human) P01133, (Rat) P07522

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Entrez Gene ID: (Human) 1950, (Rat) 25313

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protein tyrosine kinase activity Ras guanyl-nucleotide exchange factor activity epidermal growth factor receptor binding calcium ion binding protein binding growth factor activity Wnt-protein binding transmembrane receptor protein tyrosine kinase activator activity Wnt-activated receptor activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity transmembrane signal receptor

MAPK cascade activation of MAPKK activity activation of MAPK activity angiogenesis platelet degranulation DNA replication signal transduction activation of transmembrane receptor protein tyrosine kinase activity epidermal growth factor receptor signaling pathway STAT protein import into nucleus positive regulation of cell proliferation positive regulation of peptidyl-threonine phosphorylation regulation of phosphatidylinositol 3-kinase signaling peptidyl-tyrosine phosphorylation positive regulation of cerebellar granule cell precursor proliferation positive regulation of catenin import into nucleus ERBB2 signaling pathway negative regulation of epidermal growth factor receptor signaling pathway positive regulation of phosphorylation positive regulation of DNA binding positive regulation of MAP kinase activity positive regulation of GTPase activity Wnt signaling pathway involved in dorsal/ventral axis specification positive regulation of epidermal growth factor-activated receptor activity positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling branching morphogenesis of an epithelial tube negative regulation of secretion canonical Wnt signaling pathway mammary gland alveolus development ERK1 and ERK2 cascade regulation of calcium ion import negative regulation of cholesterol efflux positive regulation of hyaluronan biosynthetic process regulation of protein localization to cell surface positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process regulation of cell motility intracellular signal transduction regulation of protein secretion regulation of peptidyl-tyrosine phosphorylation regulation of protein transport