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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
MA3-030 detects brain nitric oxide synthase (bNOS), inducible NOS (iNOS) and epithelial NOS (eNOS) in bovine, human, mouse and rat tissues.
MA3-030 has been successfully used in Western blot, immunofluorescence, immunoprecipitation, and immunohistochemical experiments. By Western blot, this antibody detects an ~130 kDa band representing iNOS in samples first induced with interferon (IFN) and lipopolysaccarides (LPS), an ~155 kDa band in tissues expressing bNOS and an ~140 kDa band in tissues expressing eNOS. Immunohistochemical staining of NOS with MA3-030 yields a pattern consistent with that seen in the literature and depends on the tissue being studied and the localization of the isoforms present.
The MA3-030 antigen is purified bovine bNOS.
NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti-tumor and anti-pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); induciblr NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS). This antibody reacts with iNOS, bNOS and eNOS.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: calcium-calmodulin independent nitric oxide synthase iNOS; cNOS; constitutive endothelial nitric oxide synthase; constitutive NOS; EC-NOS; endothelial nitric oxide synthase; endothelial nitric oxide synthase 3; endothelial NOS; eNOS; hepatocyte NOS; inducible nitric oxide synthase; inducible NO synthase; inducible NOS; macrophage NOS; N-NOS; NC-NOS; neuronal nitric oxide synthase; neuronal NOS; nitric oxidase synthase; nitric oxide synthase; nitric oxide synthase 1 (neuronal); nitric oxide synthase 1, neuronal; nitric oxide synthase 2, inducible; nitric oxide synthase 2, inducible, macrophage; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3, endothelial cell; nitric oxide synthase, brain; nitric oxide synthase, endothelial; nitric oxide synthase, inducible-like; nitric oxide synthase, macrophage; nNOS; nNOS mu; NOS type I; NOS type II; NOS type III; NOS, type II; Nos3; NOSII; NOSIII; peptidyl-cysteine S-nitrosylase NOS1; peptidyl-cysteine S-nitrosylase NOS2; unnamed protein product
基因别名: 2310005C01Rik; 2310065A03Rik; bNOS; cNOS; EC-NOS; ECNOS; eNOS; HEP-NOS; i-NOS; IHPS1; INOS; MAC-NOS; MYMY8; N-NOS; NC-NOS; nNOS; NO; NOS; Nos-1; Nos-2; Nos-3; NOS-I; NOS-II; NOS2A; NOSIII
UniProt ID: (Human) P29475, (Human) P35228, (Bovine) Q27995, (Human) P29474, (Mouse) Q9Z0J4, (Rat) P29476, (Rat) Q06518, (Mouse) P29477, (Mouse) P70313, (Rat) Q62600
Entrez Gene ID: (Human) 4842, (Human) 4843, (Bovine) 282876, (Bovine) 287024, (Human) 4846, (Mouse) 18125, (Rat) 24598, (Rat) 24599, (Mouse) 18126, (Mouse) 18127, (Rat) 24600