Search Thermo Fisher Scientific
Invitrogen
20-Hydroxyecdysone ELISA Kit
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
产品规格
检测灵敏度
197 pg/mL
测定范围
102.4-25,000 pg/mL
检测样本类型/体积
{Serum=50 µL, Plasma=50 µL, Fecal Extract=50 µL, Urine=12.5 µL, Supernatant=50 µL}
手工操作时间
45 min
总时间
2 hr 30 min
均相（无需洗涤）
No
批间差异
9%
批内差异
10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Anti-IgG coated 96 well plate
Standard
Assay Buffer Concentrate
Capture Antibody
HRP-conjugated Antigen
Wash Buffer Concentrate
TMB Substrate
Stop Solution
Plate Sealer
Standard
Assay Buffer Concentrate
Capture Antibody
HRP-conjugated Antigen
Wash Buffer Concentrate
TMB Substrate
Stop Solution
Plate Sealer
运输条件
Dry ice
储存
-20°C
蛋白名称
20-Hydroxyecdysone
均相（无需洗涤）
Human
检测试剂盒格式
Competitive ELISA
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The 20-Hydroxyecdysone Competitive ELISA quantitates 20-Hydroxyecdysone (20E) in serum, plasma, urine, saliva, dried fecal extracts or cell culture medium.|原理方法: The 20-Hydroxyecdysone Competitive ELISA research-use-only kit is designed to quantitatively measure 20-Hydroxyecdysone independent of species. A 20-Hydroxyecdysone standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with anti-rabbit IgG antibodies. Anti-rabbit 20-Hydroxyecdysone antibodies and 20-Hydroxyecdysone-peroxidase conjugate is added to the wells. The 20-Hydroxyecdysone-peroxidase conjugate and any 20-Hydroxyecdysone in the sample will compete to bind to the anti-rabbit 20-Hydroxyecdysone antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound 20-Hydroxyecdysone-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of 20-Hydroxyecdysone present in the sample.|Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
20-Hydroxyecdysone is the most widely occurring ecdysteroid in both plant and animal species. It controls cell death during metamorphosis of Drosophila melanogaster.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名 : ecdysterone, 20E, C27H44O7
