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Invitrogen
Bovine Cortisol Rapid ELISA Kit
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产品规格
检测灵敏度
2.54 ng/mL
测定范围
6.25-400 ng/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
Cortisol
均相（无需洗涤）
Bovine
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Bovine Cortisol ELISA quantitates Cortisol in serum and plasma.
原理方法
The Bovine Cortisol solid-phase competitive ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Cortisol in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
Bovine Cortisol in samples (or standards) competes with a fixed amount of Cortisol immobilized on the solid phase for binding to the HRP-linked detection antibody specific to Cortisol. Excess conjugate and unbound sample or standard are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The intensity of this signal, measured spectrophotometrically at a wavelength of 450 ± 2 nm, is inversely proportional to the concentration of Cortisol present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. In plasma a majority of cortisol is bound with high affinity to corticosteroid binding globulin (CBG or transcotin). Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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