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Invitrogen
Human ACE2 Rapid ELISA Kit
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产品规格
检测灵敏度
0.23 ng/mL
测定范围
0.39-25 ng/mL
检测样本类型/体积
{Serum=50 µL, Plasma=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
ACE2
均相（无需洗涤）
Human
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Human ACE2 (Angiotensin I Converting Enzyme 2) ELISA quantitates ACE2 in serum and plasma.
原理方法
The Human Angiotensin I Converting Enzyme 2 (ACE2) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Angiotensin I Converting Enzyme 2 (ACE2) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Angiotensin I Converting Enzyme 2 (ACE2). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Angiotensin I Converting Enzyme 2 (ACE2) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : ACE2, ACEH, UNQ868/PRO1885
基因ID : (Human) 59272
基因符号 : ACE2
蛋白别名 : ACE 2, ACE-related carboxypeptidase, ACE2, ACEH, angiotensin I converting enzyme (peptidyl-dipeptidase A) 2, angiotensin I converting enzyme 2, Angiotensin-converting enzyme 2, Angiotensin-converting enzyme homolog, Angiotensin-converting enzyme-related carboxypeptidase, Metalloprotease MPROT15, peptidyl-dipeptidase A, sACE2, N-terminally-truncated natural isoform, unnamed protein product, zinc metalloprotease
UniProt ID (Human) Q9BYF1
