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产品规格
检测灵敏度
0.23 ng/mL
测定范围
0.23-50 ng/mL
检测样本类型/体积
{Serum=50 µL, Plasma=50 µL, Supernatant=50 µL}
手工操作时间
1 hr 20 min
总时间
4 hr 45 min
均相（无需洗涤）
No
批间差异
<12%
批内差异
<10%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
APE1
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
Human APE1/APEX1 quantitates human APE1/APEX1 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human APE1/APEX1.
靶标信息
Mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is a multifunctional, bipartite enzyme that plays an important role in numerous, cellular functions. APE is responsible for repairing abasic sites in DNA and in regulating the redox state of other proteins that play roles in oxidative signaling, transcription factor regulation (Fos, Jun, NF-kB, Myb, HIF-1 alpha, CREB, Pax), cell cycle control (p53), and apoptosis. The most common form of DNA damage is the creation of abasic sites which are brought about through spontaneous loss or oxidative DNA damage, through chemically initiated hydrolysis (chemotherapy), ionizing radiation, UV irradiation, oxidizing agents, and removal of modified bases by DNA glycosylases. APE is differentially expressed during development and in different tissues. This protein has diverse subcellular localization patterns which support the possibility of its interaction with numerous, other cellular proteins in addition to DNA repair within the nucleus. Regulation of APE by phosphorylation is mediated, at least in part, by casein kinase II. Increases in APE message and protein levels are observed upon the reintroduction of oxygen to hypoxic cells, and in some malignant tissue relative to normal tissue. Decreases in APE expression have been associated with the induction of cellular apoptosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : APE, APE1, APEN, APEX, APEX1, APX, HAP1, REF1
基因ID : (Human) 328
基因符号 : APEX1
蛋白别名 : AP endonuclease 1, AP endonuclease class I, AP lyase, APEN, APEX nuclease, APEX nuclease (multifunctional DNA repair enzyme) 1, Apurinic-apyrimidinic endonuclease 1, apurinic/apyrimidinic (abasic) endonuclease, deoxyribonuclease (apurinic or apyrimidinic), DNA repair nuclease/redox regulator APEX1, DNA-(apurinic or apyrimidinic site) endonuclease, protein REF-1, Redox factor-1, REF-1
UniProt ID (Human) P27695
