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Invitrogen
Human Angiotensin 2 ELISA Kit
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产品规格
检测灵敏度
18.75 pg/mL
测定范围
31.25-2,000 pg/mL
检测样本类型/体积
{Serum=50 µL, Plasma=50 µL}
手工操作时间
1 hr 20 min
总时间
2 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
Angiotensin II
蛋白家族
Serpin
均相（无需洗涤）
Human
检测试剂盒格式
Competitive ELISA
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human Angiotensin II (Ang-II) ELISA quantitates Ang-II in serum, plasma and other biological fluids
原理方法:
The Competitive Angiotensin II (Ang-II) ELISA research-use-only kit is designed to quantitatively measure Ang-II in Humans. An Ang-II standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to Human Ang-II is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of Human Ang-II in the samples is then determined by comparing the OD of the samples to the standard curve.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : AGT, ANHU, SERPINA8
基因ID : (Human) 183
基因符号 : AGT
蛋白别名 : alpha-1 antiproteinase, antitrypsin, angiotensin I, angiotensin II, Angiotensinogen, angiotensinogen (serpin peptidase inhibitor, clade A, member 8), FLJ92595, FLJ97926, pre-angiotensinogen, serine (or cysteine) proteinase inhibitor, Serpin A8, serpin peptidase inhibitor, member 8
UniProt ID (Human) P01019
