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The Human Aspartate Aminotransferase (AST) ELISA quantitates Aspartate Aminotransferase in serum, plasma, and other biological fluids
The Human Aspartate Aminotransferase solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
GOT1 is a glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
基因别名 : AST1, ASTQTL1, cAspAT, cCAT, GIG18, GOT1
基因ID : (Human) 2805
基因符号 : GOT1
蛋白别名 : aspartate aminotransferase 1, Aspartate aminotransferase, cytoplasmic, aspartate transaminase 1, cAspAT, cCAT, Cysteine aminotransferase, Cysteine transaminase, Glutamate oxaloacetate transaminase 1, glutamic-oxaloacetic transaminase 1, soluble, growth-inhibiting protein 18, testis secretory sperm-binding protein Li 196a, Transaminase A
UniProt ID (Human) P17174