Search
Invitrogen
Human E-selectin (Soluble) Instant ELISA™ Kit
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
产品规格
检测灵敏度
0.26 ng/mL
测定范围
1.56-50 ng/mL
检测样本类型/体积
{Plasma=20 µL, Serum=20 µL, Supernatant=20 µL}
手工操作时间
15 min
总时间
3 hr 10 min
均相（无需洗涤）
No
批间差异
6.2%
批内差异
8.7%
检测仪器
Absorbance Microplate Reader
产品规格
128 Tests
产品成分
Pre-coated 96 well Microplate with HRP-Conjugate and Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Adhesive Plate Covers
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Adhesive Plate Covers
运输条件
Dry ice
储存
-20°C
蛋白名称
E-selectin (CD62E)
均相（无需洗涤）
Human
检测试剂盒格式
Instant ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human Soluble Endothelial Leukocyte Adhesion Molecule-1 (ELAM-1) (Hu E-selectin) ELISA quantitates Hu sE-Selectin in human serum, plasma, cell culture supernatants, urine, amniotic fluid, bile, or other body fluids. The assay will exclusively recognize both natural and recombinant Hu sE-Selectin.
原理方法
The Human sE-Selectin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
基因别名 : CD62E, ELAM, ELAM1, ESEL, LECAM2, SELE
基因ID : (Human) 6401
基因符号 : SELE
蛋白别名 : CD62 antigen-like family member E, CD62E, e selectin, RP1-117P20.2, E-selectin, ELAM-1, endothelial adhesion molecule 1, Endothelial leukocyte adhesion molecule 1, LECAM2, leukocyte endothelial cell adhesion molecule 2, Leukocyte-endothelial cell adhesion molecule 2
UniProt ID (Human) P16581
