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Invitrogen
Human GDF15 Rapid ELISA Kit
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产品规格
检测灵敏度
18.75 pg/mL
测定范围
31.25-2000 pg/mL
检测样本类型/体积
{Serum=50 µL, Plasma=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
GDF15
均相（无需洗涤）
Human
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Human GDF15 (Growth Differentiation Factor 15) ELISA quantitates GDF15 in serum and plasma.
原理方法
The Human Growth Differentiation Factor 15 (GDF15) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Growth Differentiation Factor 15 (GDF15) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Growth Differentiation Factor 15 (GDF15). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Growth Differentiation Factor 15 (GDF15) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
GDF-15 belongs to the TGF-beta cytokine family, whose members play an important role during prenatal development and postnatal growth, and the remodeling and maintenance of a variety of tissues and organs. GDF-15 is expressed predominantly in the placenta and, to a much lesser extent, in various other tissues. The presence of GDF-15 in amniotic fluid and its elevated levels in the sera of pregnant women suggest GDF-15's involvement in gestation and embryonic development. GDF-15 generally exerts tumor suppressive activities and is one of the predominant factors produced and secreted in response to activation of the p53 pathway. Interestingly, the serum level of GDF-15 is positively correlated with neoplastic progression of several tumor types, including certain colorectal, pancreatic, and prostate cancers.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : GDF-15, GDF15, HG, MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB
基因ID : (Human) 9518
基因符号 : GDF15
蛋白别名 : GDF-15, Growth/differentiation factor 15, Macrophage inhibitory cytokine 1, MIC-1, NAG-1, NAG1, non-steroidal anti-inflammatory drug-activated gene-1, NRG-1, NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1, NSAID-activated gene 1 protein, NSAID-regulated gene 1 protein, PDF, Placental bone morphogenetic protein, Placental TGF-beta, Prostate differentiation factor, PTGF-beta, TGF-b superfamily cytokine, unnamed protein product
UniProt ID (Human) Q99988
