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Invitrogen
Human GROa Rapid ELISA Kit
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Product Specifications
Analytical sensitivity
0.09 ng/mL
Assay range
0.16-10 ng/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Hands-on time
15 min
Time-to-result
1 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
CXCL1 (GRO alpha)
Species (tested)
Human
Assay kit format
Rapid ELISA Kit
Detector antibody conjugate
HRP
Label or dye
Unconjugated
About This Kit
The Human CXCL1 (Growth Regulated Oncogene Alpha) ELISA quantitates CXCL1 in serum and plasma.
Principle of the method
The Human Growth Regulated Oncogene Alpha (CXCL1) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Growth Regulated Oncogene Alpha (CXCL1) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Growth Regulated Oncogene Alpha (CXCL1). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Growth Regulated Oncogene Alpha (CXCL1) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CXCL1, FSP, GRO, GRO1, GROA, MGSA, MGSA-a, NAP-3, SCYB1
Gene ID : (Human) 2919
Gene symbol : CXCL1
Protein Aliases : C Cmotif chemokine, C X C motif chemokine, CC motif chemokine, CCmotif chemokine, CXC, GROα, NAP3, CXC motif chemokine, CXCL, CXCL-1, GRO a, GRO α, GROalpha, C-X-C motif chemokine 1, chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha), fibroblast secretory protein, gro protein, GRO-alpha(1-73), GRO1 oncogene (melanoma growth stimulating activity, GRO1 oncogene (melanoma growth-stimulating activity), Growth-regulated alpha protein, melanoma growth stimulating activity, alpha, Melanoma growth stimulatory activity, melanoma growth stimulatory activity alpha, MGSA, MGSA alpha, NAP-3, Neutrophil-activating protein 3, unnamed protein product
UniProt ID (Human) P09341
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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