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Invitrogen
Human IL-35 ELISA Kit
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产品规格
检测灵敏度
9.38 pg/mL
测定范围
15.63-1,000 pg/mL
检测样本类型/体积
{Serum=100 µL, Plasma=100 µL}
手工操作时间
1 hr 20 min
总时间
3 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
IL-35
蛋白家族
IL-12
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human IL-35 ELISA quantitates IL-35 in serum, plasma, and other biological fluids
The Human IL-35 (Interleukin 35) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
IL-35, or Interleukin 35 is a cytokine belonging to the IL12 family, that is produced by regulatory T cells. It is a dimeric protein composed of IL-12-alpha and IL-27-beta chains. Members of this class of cytokines are important in influencing T helper cell differentiation and supressing inflammatory response. IL-35 is known to induce proliferation of regulatory T cells, and reduce the activity of helper Th17 cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : CLMF, IL-12A, IL12A, NFSK, NKSF1, P35
基因ID : (Human) 3592
基因符号 : IL12A
蛋白别名 : CLMF p35, cytotoxic lymphocyte maturation factor 1, p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, IL-12, subunit p35, IL-12A, IL12p35, il35, ILN, Interleukin, Interleukin-35, IL35 subunit, interleukin 12, interleukin 12A (natural killer cell stimulatory factor 1, p35), interleukin-12 alpha chain, Interleukin-12 subunit alpha, NF cell stimulatory factor chain 1, NK cell stimulatory factor chain 1, NKSF1
UniProt ID (Human) P29459
