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The Human Mucin 2 (MUC2) ELISA quantitates Mucin 2 in serum, plasma, and other biological fluids
The Human Mucin 2 (MUC2) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Secreted epithelial mucins are large macromolecules which exhibit extreme polydispersity. Mucin 2 is the major intestinal mucin. O-glycans are attached to MUC2 in a potentially diverse arrangement, which is crucial for their interaction with endogeneous and exogeneous lectins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
基因别名 : MLP, MUC-2, SMUC
基因ID : (Human) 4583
基因符号 : MUC2
蛋白别名 : intestinal mucin, MUC2, mucin 2, intestinal/tracheal, mucin-like protein
UniProt ID (Human) Q02817