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The Mouse ApoA1 (Apolipoprotein A1) ELISA quantitates ApoA1 in serum, plasma, and other biological fluids
The Mouse ApoA1 (Apolipoprotein A1) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 270-380 mg/100 mL.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
基因别名 : Alp-1, apo-AI, Apoa-1, apoA-I, Apoa1, Brp-14, Ltw-1, Lvtw-1, Sep-1, Sep-2, Sep2
基因ID : (Mouse) 11806
基因符号 : Apoa1
蛋白别名 : Apo-AI, Apolipoprotein A-I, Apolipoprotein A1
UniProt ID (Mouse) Q00623