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The mouse Interleukin-6 (IL-6) ELISA kit is a valuable tool for the precise measurement of mouse IL-6 in various biological samples, such as murine serum, plasma, buffered solutions, or cell culture medium. This highly specific assay is specifically designed to exclusively detect and quantify both endogenous and recombinant forms of mouse IL-6.
原理方法
Employing a well-matched pair of antibodies, the mouse IL-6 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) accurately quantifies the target analyte. The microplate wells are pre-coated with a target-specific antibody, facilitating the binding of the analyte during the assay. Upon the addition of samples or standards they selectively bind to the immobilized (capture) antibody within the wells. The formation of a sandwich complex is accomplished by introducing a second (detector) antibody. Subsequently, a substrate solution is added, initiating a reaction with the enzyme-antibody-target complex and generating a measurable signal. The intensity of this signal directly correlates with the concentration of the target present in the original specimen.
IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
基因别名 : Il-6, Il6
基因ID : (Mouse) 16193
基因符号 : Il6
蛋白别名 : B-cell hybridoma growth factor, IL-6, Interleukin HP-1, H-IL-6, ILN, Interleukin, Interleukin6, M-IL-6, R-IL-6, Interleukin-6
UniProt ID (Mouse) P08505