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Invitrogen
Mouse IL-13 Matched Antibody Pair
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产品规格
检测灵敏度
7.8 pg/mL
测定范围
7.8-500 pg/mL
检测样本类型/体积
{Plasma=100 µL, Serum=100 µL, Supernatant=100 µL}
手工操作时间
1 hr 30 min
总时间
24 hr
均相（无需洗涤）
No
检测仪器
Absorbance Microplate Reader
产品规格
10 x 96 Tests
产品成分
Capture (coating) Antibody
Detection Antibody
Standard
Streptavidin-HRP
Detection Antibody
Standard
Streptavidin-HRP
运输条件
Wet ice
储存
2-8°C
蛋白名称
IL-13
Target site-specificity
检测试剂盒格式
Matched Antibody Pair
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Mouse IL-13 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-13. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate.
原理方法
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
靶标信息
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名 : IL-13, T-cell activation protein P600
