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Invitrogen
Mouse SDF-1 alpha/CXCL12 ELISA Kit
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产品规格
检测灵敏度
3 pg/mL
测定范围
3-600 pg/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL, Supernatant=100 µL}
手工操作时间
1 hr 20 min
总时间
4 hr 45 min
均相（无需洗涤）
No
批间差异
<12%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
蛋白名称
CXCL12
蛋白家族
Chemokines & Receptors
均相（无需洗涤）
Mouse
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Mouse SDF-1 alpha/CXCL12 ELISA quantitates Ms SDF-1α in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms SDF-1α.
原理方法
The Mouse SDF-1α solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
生物信息学
基因别名 : Pbsf, Scyb12, Sdf1, Tlsf, Tpar1
基因ID : (Mouse) 20315
基因符号 : Cxcl12
蛋白别名 : 12-O-tetradecanoylphorbol 13-acetate repressed protein 1, AI174028, C Cmotif chemokine, C X C motif chemokine, CC motif chemokine, CCmotif chemokine, CXC, stromal cell-derived factor-1 alpha, Tlsfa, TLSF-a, Tlsfb, TLSF-b, CXC motif chemokine, CXCL, hIRH, hSDF-1, SDF-1a, SDF-1b, chemokine (C-X-C motif) ligand 12, Cxcl12, interleukin-8 homolog, pre-B-cell growth-stimulating factor, thymic lymphoma cell-stimulating factor, unnamed protein product
UniProt ID (Mouse) P40224
